Title |
Analysis of fluorescent reporters indicates heterogeneity in glucose uptake and utilization in clonal bacterial populations
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Published in |
BMC Microbiology, November 2013
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DOI | 10.1186/1471-2180-13-258 |
Pubmed ID | |
Authors |
Nela Nikolic, Thomas Barner, Martin Ackermann |
Abstract |
In this study, we aimed at investigating heterogeneity in the expression of metabolic genes in clonal populations of Escherichia coli growing on glucose as the sole carbon source. Different metabolic phenotypes can arise in these clonal populations through variation in the expression of glucose transporters and metabolic enzymes. First, we focused on the glucose transporters PtsG and MglBAC to analyze the diversity of glucose uptake strategies. Second, we analyzed phenotypic variation in the expression of genes involved in gluconeogenesis and acetate scavenging (as acetate is formed and excreted during bacterial growth on glucose), which can reveal, for instance, phenotypic subpopulations that cross-feed through the exchange of acetate. In these experiments, E. coli MG1655 strains containing different transcriptional GFP reporters were grown in chemostats and reporter expression was measured with flow cytometry. |
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Geographical breakdown
Country | Count | As % |
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Spain | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
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France | 1 | <1% |
Sweden | 1 | <1% |
United Kingdom | 1 | <1% |
Mexico | 1 | <1% |
China | 1 | <1% |
Estonia | 1 | <1% |
United States | 1 | <1% |
Unknown | 127 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 34 | 25% |
Researcher | 22 | 16% |
Student > Master | 17 | 13% |
Student > Bachelor | 9 | 7% |
Lecturer | 7 | 5% |
Other | 24 | 18% |
Unknown | 21 | 16% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 44 | 33% |
Biochemistry, Genetics and Molecular Biology | 27 | 20% |
Immunology and Microbiology | 12 | 9% |
Engineering | 8 | 6% |
Mathematics | 3 | 2% |
Other | 12 | 9% |
Unknown | 28 | 21% |