Title |
Expression of inflammation-related genes is associated with adipose tissue location in horses
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Published in |
BMC Veterinary Research, December 2013
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DOI | 10.1186/1746-6148-9-240 |
Pubmed ID | |
Authors |
Lien Bruynsteen, Tim Erkens, Luc J Peelman, Richard Ducatelle, Geert PJ Janssens, Patricia A Harris, Myriam Hesta |
Abstract |
In humans, adipose tissue (AT) originating from different depots shows varying gene expression profiles. In horses, the risk of certain metabolic disorders may also be influenced by the impact of specific AT depots. Macrophage infiltration in human and rat AT is considered to be a source of inflammatory changes. In horses, this relationship has not been extensively studied yet. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR), a useful method to evaluate differences in mRNA expression across different tissues, can be used to evaluate differences between equine AT depots. For a correct interpretation of the RT-qPCR results, expression data have to be normalized by the use of validated reference genes. The main objectives of this study were to compare mRNA expression of inflammation-related genes, as well as adipocyte morphology and number between different equine AT depots; and in addition, to investigate the presence of antigen presenting cells in equine AT and any potential relationship with adipokine mRNA expression. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 2% |
South Africa | 1 | 2% |
Unknown | 42 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 14% |
Other | 5 | 11% |
Student > Bachelor | 5 | 11% |
Researcher | 5 | 11% |
Student > Master | 4 | 9% |
Other | 9 | 20% |
Unknown | 10 | 23% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 12 | 27% |
Agricultural and Biological Sciences | 9 | 20% |
Medicine and Dentistry | 7 | 16% |
Biochemistry, Genetics and Molecular Biology | 2 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Other | 2 | 5% |
Unknown | 11 | 25% |