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Attention Score in Context
| Title |
Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs
|
|---|---|
| Published in |
Reproductive Biology and Endocrinology, November 2016
|
| DOI | 10.1186/s12958-016-0206-5 |
| Pubmed ID | |
| Authors |
Jung-Taek Kang, Bumrae Cho, Junghyun Ryu, Caitlin Ray, Eun-Jin Lee, Yun-Jin Yun, SunMi Ahn, JinSeok Lee, Dal-Young Ji, Nathaniel Jue, Sherrie Clark-Deener, Kiho Lee, Kwang-Wook Park |
| Abstract |
Pigs with SCID can be a useful model in regenerative medicine, xenotransplantation, and cancer cell transplantation studies. Utilizing genome editing technologies such as CRISPR/Cas9 system allows us to generate genetically engineered pigs at a higher efficiency. In this study, we report generation and phenotypic characterization of IL2RG knockout female pigs produced through combination of CRISPR/Cas9 system and SCNT. As expected, pigs lacking IL2RG presented SCID phenotype. First, specific CRISPR/Cas9 systems targeting IL2RG were introduced into developing pig embryos then the embryos were transferred into surrogates. A total of six fetuses were obtained from the embryo transfer and fetal fibroblast cell lines were established. Then IL2RG knockout female cells carrying biallelic genetic modification were used as donor cells for SCNT, followed by embryo transfer. Three live cloned female piglets carrying biallelic mutations in IL2RG were produced. All cloned piglets completely lacked thymus and they had a significantly reduced level of mature T, B and NK cells in their blood and spleen. Here, we generated IL2RG knockout female pigs showing phenotypic characterization of SCID. This IL2RG knockout female pigs will be a promising model for biomedical and translational research. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 14% |
| Student > Doctoral Student | 3 | 11% |
| Student > Bachelor | 3 | 11% |
| Researcher | 3 | 11% |
| Professor | 2 | 7% |
| Other | 6 | 21% |
| Unknown | 7 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 25% |
| Agricultural and Biological Sciences | 7 | 25% |
| Medicine and Dentistry | 5 | 18% |
| Social Sciences | 1 | 4% |
| Engineering | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 6 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 May 2017.
All research outputs
#18,546,002
of 22,968,808 outputs
Outputs from Reproductive Biology and Endocrinology
#670
of 982 outputs
Outputs of similar age
#235,987
of 312,084 outputs
Outputs of similar age from Reproductive Biology and Endocrinology
#7
of 12 outputs
Altmetric has tracked 22,968,808 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 982 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 10.0. This one is in the 14th percentile – i.e., 14% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 312,084 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 33rd percentile – i.e., 33% of its contemporaries scored the same or lower than it.