Title |
Marker-trait association analysis of functional gene markers for provitamin A levels across diverse tropical yellow maize inbred lines
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Published in |
BMC Plant Biology, December 2013
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DOI | 10.1186/1471-2229-13-227 |
Pubmed ID | |
Authors |
Girum Azmach, Melaku Gedil, Abebe Menkir, Charles Spillane |
Abstract |
Biofortification of staple crops is a cost effective and sustainable approach that can help combat vitamin A and other micronutrient deficiencies in developing countries. PCR -based DNA markers distinguishing alleles of three key genes of maize endosperm carotenoid biosynthesis (PSY1, lcyE and crtRB1) have been developed to facilitate maize provitamin A biofortification via marker assisted selection. Previous studies of these functional DNA markers revealed inconsistent effects. The germplasm previously employed for discovering and validating these functional markers was mainly of temperate origin containing low frequencies of the favourable allele of the most significant polymorphism, crtRB1-5'TE. Here, we investigate the vitamin A biofortification potential of these DNA markers in a germplasm panel of diverse tropical yellow maize inbred lines, with mixed genetic backgrounds of temperate and tropical germplasm to identify the most effective diagnostic markers for vitamin A biofortification. |
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