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Efficient itaconic acid production from glycerol with Ustilago vetiveriae TZ1

Overview of attention for article published in Biotechnology for Biofuels, May 2017
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (75th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (60th percentile)

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1 tweeter
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2 patents

Citations

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31 Dimensions

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43 Mendeley
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Title
Efficient itaconic acid production from glycerol with Ustilago vetiveriae TZ1
Published in
Biotechnology for Biofuels, May 2017
DOI 10.1186/s13068-017-0809-x
Pubmed ID
Authors

Thiemo Zambanini, Hamed Hosseinpour Tehrani, Elena Geiser, Dorothee Merker, Sarah Schleese, Judith Krabbe, Joerg M. Buescher, Guido Meurer, Nick Wierckx, Lars M. Blank

Abstract

The family of Ustilaginaceae is known for their capability to naturally produce industrially valuable chemicals from different carbon sources. Recently, several Ustilaginaceae were reported to produce organic acids from glycerol, which is the main side stream in biodiesel production. In this study, we present Ustilago vetiveriae as new production organism for itaconate synthesis from glycerol. In a screening of 126 Ustilaginaceae, this organism reached one of the highest titers for itaconate combined with a high-glycerol uptake rate. By adaptive laboratory evolution, the production characteristics of this strain could be improved. Further medium optimization with the best single colony, U. vetiveriae TZ1, in 24-deep well plates resulted in a maximal itaconate titer of 34.7 ± 2.5 g L(-1) produced at a rate of 0.09 ± 0.01 g L(-1) h(-1) from 196 g L(-1) glycerol. Simultaneously, this strain produced 46.2 ± 1.4 g L(-1) malate at a rate of 0.12 ± 0.00 g L(-1) h(-1). Due to product inhibition, the itaconate titer in NaOH-titrated bioreactor cultivations was lower (24 g L(-1)). Notably, an acidic pH value of 5.5 resulted in decreased itaconate production, however, completely abolishing malate production. Overexpression of ria1 or mtt1, encoding a transcriptional regulator and mitochondrial transporter, respectively, from the itaconate cluster of U. maydis resulted in a 2.0-fold (ria1) and 1.5-fold (mtt1) higher itaconate titer in comparison to the wild-type strain, simultaneously reducing malate production by 75 and 41%, respectively. The observed production properties of U. vetiveriae TZ1 make this strain a promising candidate for microbial itaconate production. The outcome of the overexpression experiments, which resulted in reduced malate production in favor of an increased itaconate titer, clearly strengthens its potential for industrial itaconate production from glycerol as major side stream of biodiesel production.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 43 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 10 23%
Student > Master 9 21%
Student > Ph. D. Student 7 16%
Student > Doctoral Student 3 7%
Researcher 3 7%
Other 6 14%
Unknown 5 12%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 33%
Engineering 6 14%
Agricultural and Biological Sciences 5 12%
Environmental Science 2 5%
Immunology and Microbiology 2 5%
Other 5 12%
Unknown 9 21%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 December 2019.
All research outputs
#2,884,929
of 16,374,171 outputs
Outputs from Biotechnology for Biofuels
#215
of 1,197 outputs
Outputs of similar age
#62,846
of 271,165 outputs
Outputs of similar age from Biotechnology for Biofuels
#2
of 5 outputs
Altmetric has tracked 16,374,171 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,197 research outputs from this source. They receive a mean Attention Score of 4.4. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 271,165 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.