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Super-resolution imaging with Pontamine Fast Scarlet 4BS enables direct visualization of cellulose orientation and cell connection architecture in onion epidermis cells

Overview of attention for article published in BMC Plant Biology, December 2013
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Title
Super-resolution imaging with Pontamine Fast Scarlet 4BS enables direct visualization of cellulose orientation and cell connection architecture in onion epidermis cells
Published in
BMC Plant Biology, December 2013
DOI 10.1186/1471-2229-13-226
Pubmed ID
Authors

Johannes Liesche, Iwona Ziomkiewicz, Alexander Schulz

Abstract

In plants, a complex cell wall protects cells and defines their shape. Cellulose fibrils form a multilayered network inside the cell-wall matrix that plays a direct role in controlling cell expansion. Resolving the structure of this network will allow us to comprehend the relationship of cellulose fibril orientation and growth.The fluorescent dye Pontamine Fast Scarlet 4BS (PFS) was shown to stain cellulose with high specificity and could be used to visualize cellulose bundles in cell walls of Arabidopsis root epidermal cells with confocal microscopy. The resolution limit of confocal microscopy of some 200 nm in xy and 550 nm in z for green light, restricts the direct visualization of cellulose to relatively large bundles, whereas the structure of cellulose microfibrils with their diameter below 10 nm remains unresolved. Over the last decade, several so-called super-resolution microscopy approaches have been developed; in this paper we explore the potential of such approaches for the direct visualization of cellulose.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 137 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 3 2%
Germany 2 1%
Argentina 1 <1%
Brazil 1 <1%
Unknown 130 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 33 24%
Researcher 24 18%
Student > Master 13 9%
Student > Doctoral Student 11 8%
Student > Bachelor 9 7%
Other 22 16%
Unknown 25 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 59 43%
Biochemistry, Genetics and Molecular Biology 16 12%
Chemistry 7 5%
Engineering 7 5%
Physics and Astronomy 6 4%
Other 14 10%
Unknown 28 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 January 2014.
All research outputs
#19,942,887
of 25,371,288 outputs
Outputs from BMC Plant Biology
#2,175
of 3,587 outputs
Outputs of similar age
#234,293
of 319,345 outputs
Outputs of similar age from BMC Plant Biology
#35
of 58 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,587 research outputs from this source. They receive a mean Attention Score of 3.0. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 319,345 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.