CRISPR transcript processing: a mechanism for generating a large number of small interfering RNAs.
Biology Direct, July 2012
CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated sequences) is a recently discovered prokaryotic defense system against foreign DNA, including viruses and plasmids. CRISPR cassette is transcribed as a continuous transcript (pre-crRNA), which is processed by Cas proteins into small RNA molecules (crRNAs) that are responsible for defense against invading viruses. Experiments in E. coli report that overexpression of cas genes generates a large number of crRNAs, from only few pre-crRNAs.
|Readers by professional status||Count||As %|
|Student > Ph. D. Student||21||28%|
|Student > Master||7||9%|
|Professor > Associate Professor||5||7%|
|Student > Bachelor||5||7%|
|Readers by discipline||Count||As %|
|Agricultural and Biological Sciences||42||57%|
|Biochemistry, Genetics and Molecular Biology||15||20%|
|Physics and Astronomy||2||3%|
|Immunology and Microbiology||1||1%|