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Aneuploidy screening of embryonic stem cell clones by metaphase karyotyping and droplet digital polymerase chain reaction

Overview of attention for article published in BMC Molecular and Cell Biology, August 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#21 of 1,233)
  • High Attention Score compared to outputs of the same age (90th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

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2 news outlets
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Citations

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28 Dimensions

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51 Mendeley
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Title
Aneuploidy screening of embryonic stem cell clones by metaphase karyotyping and droplet digital polymerase chain reaction
Published in
BMC Molecular and Cell Biology, August 2016
DOI 10.1186/s12860-016-0108-6
Pubmed ID
Authors

Gemma F. Codner, Loic Lindner, Adam Caulder, Marie Wattenhofer-Donzé, Adam Radage, Annelyse Mertz, Benjamin Eisenmann, Joffrey Mianné, Edward P. Evans, Colin V. Beechey, Martin D. Fray, Marie-Christine Birling, Yann Hérault, Guillaume Pavlovic, Lydia Teboul

Abstract

Karyotypic integrity is essential for the successful germline transmission of alleles mutated in embryonic stem (ES) cells. Classical methods for the identification of aneuploidy involve cytological analyses that are both time consuming and require rare expertise to identify mouse chromosomes. As part of the International Mouse Phenotyping Consortium, we gathered data from over 1,500 ES cell clones and found that the germline transmission (GLT) efficiency of clones is compromised when over 50 % of cells harbour chromosome number abnormalities. In JM8 cells, chromosomes 1, 8, 11 or Y displayed copy number variation most frequently, whilst the remainder generally remain unchanged. We developed protocols employing droplet digital polymerase chain reaction (ddPCR) to accurately quantify the copy number of these four chromosomes, allowing efficient triage of ES clones prior to microinjection. We verified that assessments of aneuploidy, and thus decisions regarding the suitability of clones for microinjection, were concordant between classical cytological and ddPCR-based methods. Finally, we improved the method to include assay multiplexing so that two unstable chromosomes are counted simultaneously (and independently) in one reaction, to enhance throughput and further reduce the cost. We validated a PCR-based method as an alternative to classical karyotype analysis. This technique enables laboratories that are non-specialist, or work with large numbers of clones, to precisely screen ES cells for the most common aneuploidies prior to microinjection to ensure the highest level of germline transmission potential. The application of this method allows early exclusion of aneuploid ES cell clones in the ES cell to mouse conversion process, thus improving the chances of obtaining germline transmission and reducing the number of animals used in failed microinjection attempts. This method can be applied to any other experiments that require accurate analysis of the genome for copy number variation (CNV).

X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 51 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 2%
Unknown 50 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 24%
Student > Ph. D. Student 9 18%
Other 7 14%
Student > Bachelor 2 4%
Student > Doctoral Student 2 4%
Other 5 10%
Unknown 14 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 27%
Agricultural and Biological Sciences 7 14%
Medicine and Dentistry 5 10%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Engineering 2 4%
Other 3 6%
Unknown 18 35%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 18. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 March 2023.
All research outputs
#1,981,199
of 25,374,647 outputs
Outputs from BMC Molecular and Cell Biology
#21
of 1,233 outputs
Outputs of similar age
#36,304
of 381,577 outputs
Outputs of similar age from BMC Molecular and Cell Biology
#1
of 19 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 92nd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,233 research outputs from this source. They receive a mean Attention Score of 4.0. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 381,577 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 19 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.