Title |
Identification of a major Listeria monocytogenes outbreak clone linked to soft cheese in Northern Italy – 2009-2011
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Published in |
BMC Infectious Diseases, May 2017
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DOI | 10.1186/s12879-017-2441-6 |
Pubmed ID | |
Authors |
Ettore Amato, Virginia Filipello, Maria Gori, Sara Lomonaco, Marina Nadia Losio, Antonio Parisi, Pol Huedo, Stephen John Knabel, Mirella Pontello |
Abstract |
Molecular subtyping and enhanced surveillance in Lombardy region identified a cluster of possibly related listeriosis cases from 2006 to 2010. This cluster grouped 31 isolates that belonged to serotype 1/2a and Sequence Type 38 (ST38) as defined by Multilocus Sequence Typing (MLST). Our study expanded the previous investigation to include cases from 2011 to 2014 and used Multi-Virulence-Locus Sequence Typing (MVLST) on all ST38 isolates to better understand their epidemiology and possibly identify a common source outbreak. Out of 306 L. monocytogenes clinical isolates collected, 43 (14.1%) belonged to ST38 with cases occurring in nine out of twelve Lombardy provinces. The ST38 isolates were split by MVLST into two Virulence Types (VTs): VT80 (n = 12) and VT104 (n = 31). VT104 cases were concentrated between 2009 and 2011 in two provinces, Bergamo and Milan. An epidemiologic investigation was performed and in one case, a matching VT104 isolate was retrieved from a soft cheese sample from a patient's refrigerator. Our findings revealed a major listeriosis outbreak in Northern Italy linked to soft cheese in 2009-2011, which went undetected by local health authorities. Our study shows that integrating subtyping methods with conventional epidemiology can help identify the source of L. monocytogenes outbreak clones. |
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Venezuela, Bolivarian Republic of | 1 | 100% |
Demographic breakdown
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 56 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 8 | 14% |
Researcher | 7 | 13% |
Student > Bachelor | 5 | 9% |
Other | 5 | 9% |
Librarian | 3 | 5% |
Other | 10 | 18% |
Unknown | 18 | 32% |
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Biochemistry, Genetics and Molecular Biology | 6 | 11% |
Immunology and Microbiology | 3 | 5% |
Medicine and Dentistry | 3 | 5% |
Other | 5 | 9% |
Unknown | 21 | 38% |