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Fermentation of lactose to ethanol in cheese whey permeate and concentrated permeate by engineered Escherichia coli

Overview of attention for article published in BMC Biotechnology, June 2017
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (68th percentile)

Mentioned by

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6 tweeters
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1 Facebook page

Citations

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33 Dimensions

Readers on

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93 Mendeley
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Title
Fermentation of lactose to ethanol in cheese whey permeate and concentrated permeate by engineered Escherichia coli
Published in
BMC Biotechnology, June 2017
DOI 10.1186/s12896-017-0369-y
Pubmed ID
Authors

Lorenzo Pasotti, Susanna Zucca, Michela Casanova, Giuseppina Micoli, Maria Gabriella Cusella De Angelis, Paolo Magni

Abstract

Whey permeate is a lactose-rich effluent remaining after protein extraction from milk-resulting cheese whey, an abundant dairy waste. The lactose to ethanol fermentation can complete whey valorization chain by decreasing dairy waste polluting potential, due to its nutritional load, and producing a biofuel from renewable source at the same time. Wild type and engineered microorganisms have been proposed as fermentation biocatalysts. However, they present different drawbacks (e.g., nutritional supplements requirement, high transcriptional demand of recombinant genes, precise oxygen level, and substrate inhibition) which limit the industrial attractiveness of such conversion process. In this work, we aim to engineer a new bacterial biocatalyst, specific for dairy waste fermentation. We metabolically engineered eight Escherichia coli strains via a new expression plasmid with the pyruvate-to-ethanol conversion genes, and we carried out the selection of the best strain among the candidates, in terms of growth in permeate, lactose consumption and ethanol formation. We finally showed that the selected engineered microbe (W strain) is able to efficiently ferment permeate and concentrated permeate, without nutritional supplements, in pH-controlled bioreactor. In the conditions tested in this work, the selected biocatalyst could complete the fermentation of permeate and concentrated permeate in about 50 and 85 h on average, producing up to 17 and 40 g/l of ethanol, respectively. To our knowledge, this is the first report showing efficient ethanol production from the lactose contained in whey permeate with engineered E. coli. The selected strain is amenable to further metabolic optimization and represents an advance towards efficient biofuel production from industrial waste stream.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 93 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 1 1%
Unknown 92 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 21 23%
Student > Ph. D. Student 15 16%
Student > Master 11 12%
Researcher 8 9%
Student > Postgraduate 5 5%
Other 11 12%
Unknown 22 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 25 27%
Biochemistry, Genetics and Molecular Biology 13 14%
Engineering 6 6%
Chemistry 5 5%
Immunology and Microbiology 4 4%
Other 16 17%
Unknown 24 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 June 2017.
All research outputs
#4,166,618
of 15,457,549 outputs
Outputs from BMC Biotechnology
#275
of 816 outputs
Outputs of similar age
#84,077
of 271,791 outputs
Outputs of similar age from BMC Biotechnology
#1
of 1 outputs
Altmetric has tracked 15,457,549 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 816 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.4. This one has gotten more attention than average, scoring higher than 66% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 271,791 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them