Title |
Reduction of meckelin leads to general loss of cilia, ciliary microtubule misalignment and distorted cell surface organization
|
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Published in |
Cilia, January 2014
|
DOI | 10.1186/2046-2530-3-2 |
Pubmed ID | |
Authors |
Tyler Picariello, Megan Smith Valentine, Junji Yano, Judith Van Houten |
Abstract |
Meckelin (MKS3), a conserved protein linked to Meckel Syndrome, assists in the migration of centrioles to the cell surface for ciliogenesis. We explored for additional functions of MKS3p using RNA interference (RNAi) and expression of FLAG epitope tagged protein in the ciliated protozoan Paramecium tetraurelia. This cell has a highly organized cell surface with thousands of cilia and basal bodies that are grouped into one or two basal body units delineated by ridges. The highly systematized nature of the P. tetraurelia cell surface provides a research model of MKS and other ciliopathies where changes in ciliary structure, subcellular organization and overall arrangement of the cell surface can be easily observed. We used cells reduced in IFT88 for comparison, as the involvement of this gene's product with cilia maintenance and growth is well understood. |
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Demographic breakdown
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Mendeley readers
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