Title |
A multi-split mapping algorithm for circular RNA, splicing, trans-splicing and fusion detection
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Published in |
Genome Biology, February 2014
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DOI | 10.1186/gb-2014-15-2-r34 |
Pubmed ID | |
Authors |
Steve Hoffmann, Christian Otto, Gero Doose, Andrea Tanzer, David Langenberger, Sabina Christ, Manfred Kunz, Lesca M Holdt, Daniel Teupser, Jörg Hackermüller, Peter F Stadler |
Abstract |
Numerous high-throughput sequencing studies focus on detecting conventionally spliced mRNAs in RNA-seq data. However, non-standard RNAs arising through gene fusion, circularization, or trans-splicing are often neglected. We introduce a novel, unbiased algorithm to detect splice junctions from single-end cDNA sequences. In contrast to other methods, our approach accommodates multi-junction structures. Our method compares favorably with competing tools on conventionally spliced mRNAs and, with a gain of up to 40\% of recall, systematically outperforms them on reads with multiple splits, trans-splicing and circular products. The algorithm is integrated into our mapping tool segemehl (www.bioinf.uni-leipzig.de/Software/segemehl/). |
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Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 6 | 21% |
United Kingdom | 4 | 14% |
Germany | 3 | 11% |
Canada | 1 | 4% |
Cameroon | 1 | 4% |
Australia | 1 | 4% |
Austria | 1 | 4% |
New Zealand | 1 | 4% |
Montenegro | 1 | 4% |
Other | 4 | 14% |
Unknown | 5 | 18% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 18 | 64% |
Members of the public | 9 | 32% |
Science communicators (journalists, bloggers, editors) | 1 | 4% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 6 | 2% |
Germany | 3 | <1% |
United Kingdom | 3 | <1% |
Denmark | 2 | <1% |
Brazil | 2 | <1% |
Ireland | 1 | <1% |
Italy | 1 | <1% |
Netherlands | 1 | <1% |
Switzerland | 1 | <1% |
Other | 6 | 2% |
Unknown | 284 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 87 | 28% |
Researcher | 73 | 24% |
Student > Master | 35 | 11% |
Student > Bachelor | 23 | 7% |
Professor > Associate Professor | 17 | 5% |
Other | 34 | 11% |
Unknown | 41 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 122 | 39% |
Biochemistry, Genetics and Molecular Biology | 74 | 24% |
Computer Science | 24 | 8% |
Medicine and Dentistry | 17 | 5% |
Engineering | 7 | 2% |
Other | 17 | 5% |
Unknown | 49 | 16% |