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Berberine regulates the protein expression of multiple tumorigenesis-related genes in hepatocellular carcinoma cell lines

Overview of attention for article published in Cancer Cell International, May 2017
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Title
Berberine regulates the protein expression of multiple tumorigenesis-related genes in hepatocellular carcinoma cell lines
Published in
Cancer Cell International, May 2017
DOI 10.1186/s12935-017-0429-3
Pubmed ID
Authors

Tung-Yueh Chuang, Hsiao-Li Wu, Jie Min, Michael Diamond, Ricardo Azziz, Yen-Hao Chen

Abstract

Hepatocellular carcinoma (HCC) is the seventh most common malignancy and the third leading cause of cancer-related death worldwide with an extremely grim prognosis. Berberine (BBR) has been found to inhibit proliferation of human HCC cells, although the underlying mechanism(s) are unclear. Protein expression was detected by Western blots. Cell viability was determined by using the CellTiter Assay kit. We confirm that BBR treatment inhibits HepG2, Hep3B, and SNU-182 cell viability, and suggest that it regulates this proliferation via the modulation of multiple tumorigenesis-related genes protein expression. BBR treatment up-regulated protein expression of tumor suppressor genes, including Kruppel-like factor 6 (KLF6), activating transcription factor 3 (ATF3) and p21, while down-regulating the expression of selected oncogenes, including E2F transcription factor 1 (E2F1) and pituitary tumor transforming gene 1 (PTTG1). The specific extracellular signal-regulated kinases 1/2 (ERK1/2) inhibitor, PD98059, partially inhibited BBR effects including reduction of cell viability, and up-regulation of KLF6 and ATF3 expressions; although, PD98059 did not alter the down-regulation of E2F1 and PTTG1 expression by BBR. Our results suggest that BBR inhibits HCC cell viability by modulating multiple tumorigenesis-related genes, and that up-regulation of tumor suppressor genes by BBR is in part the result of ERK1/2 action. The results of this study augment our understanding of the mechanisms underlying the effect of BBR on hepatocellular cancers and provide further evidence as to the biological plausibility of this agent's role in the treatment of these malignancies.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 22%
Student > Doctoral Student 2 22%
Student > Bachelor 2 22%
Professor 1 11%
Student > Master 1 11%
Other 1 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 33%
Agricultural and Biological Sciences 2 22%
Medicine and Dentistry 2 22%
Business, Management and Accounting 1 11%
Unknown 1 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 June 2017.
All research outputs
#9,127,415
of 11,403,395 outputs
Outputs from Cancer Cell International
#230
of 411 outputs
Outputs of similar age
#190,566
of 263,322 outputs
Outputs of similar age from Cancer Cell International
#4
of 7 outputs
Altmetric has tracked 11,403,395 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 411 research outputs from this source. They receive a mean Attention Score of 3.1. This one is in the 23rd percentile – i.e., 23% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,322 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 7 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.