@joachimgoedhart @KSerahSera I've also previously seen 3:1, but with the IRES from pIRES (not the pPRIG that you used in this paper). Interesting comment and extension of the pPRIG work published in 2018: https://t.co/CaH2VmhupA
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@weinberz @LaurelOld it even depends on where the AUG is - see first section of this wonderful paper: https://t.co/YSFFVg3CCw
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RT @joachimgoedhart: @LJIMicroCore @ProfBootyPhD @rita_strack @NathanShaner We compared performance of IRES and 2A for co-expression here:…
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@LJIMicroCore @ProfBootyPhD @rita_strack @NathanShaner We compared performance of IRES and 2A for co-expression here: https://t.co/3VD9sHleC7 We now prefer P2A over T2A, as P2A seems to have better separation. Note that efficiency of ORF downstream of IRES