Title |
Improving transcriptome construction in non-model organisms: integrating manual and automated gene definition in Emiliania huxleyi
|
---|---|
Published in |
BMC Genomics, February 2014
|
DOI | 10.1186/1471-2164-15-148 |
Pubmed ID | |
Authors |
Ester Feldmesser, Shilo Rosenwasser, Assaf Vardi, Shifra Ben-Dor |
Abstract |
The advent of Next Generation Sequencing technologies and corresponding bioinformatics tools allows the definition of transcriptomes in non-model organisms. Non-model organisms are of great ecological and biotechnological significance, and consequently the understanding of their unique metabolic pathways is essential. Several methods that integrate de novo assembly with genome-based assembly have been proposed. Yet, there are many open challenges in defining genes, particularly where genomes are not available or incomplete. Despite the large numbers of transcriptome assemblies that have been performed, quality control of the transcript building process, particularly on the protein level, is rarely performed if ever. To test and improve the quality of the automated transcriptome reconstruction, we used manually defined and curated genes, several of them experimentally validated. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 50% |
Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Uruguay | 2 | 2% |
Denmark | 2 | 2% |
United States | 2 | 2% |
United Kingdom | 2 | 2% |
Czechia | 1 | 1% |
Sweden | 1 | 1% |
Norway | 1 | 1% |
Taiwan | 1 | 1% |
Italy | 1 | 1% |
Other | 2 | 2% |
Unknown | 79 | 84% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 28 | 30% |
Student > Ph. D. Student | 21 | 22% |
Student > Master | 12 | 13% |
Student > Doctoral Student | 6 | 6% |
Student > Bachelor | 5 | 5% |
Other | 15 | 16% |
Unknown | 7 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 57 | 61% |
Biochemistry, Genetics and Molecular Biology | 15 | 16% |
Environmental Science | 3 | 3% |
Computer Science | 3 | 3% |
Social Sciences | 3 | 3% |
Other | 6 | 6% |
Unknown | 7 | 7% |