Title |
Rapid bioassay to measure early reactive oxygen species production in Arabidopsis leave tissue in response to living Pseudomonas syringae
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Published in |
Plant Methods, February 2014
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DOI | 10.1186/1746-4811-10-6 |
Pubmed ID | |
Authors |
John M Smith, Antje Heese |
Abstract |
Arabidopsis thaliana and Pseudomonas syringae pathovar tomato (Pto) provide an excellent plant-bacteria model system to study innate immunity. During pattern-triggered immunity (PTI), cognate host receptors perceive pathogen-associated molecular patterns (PAMPs) as non-self molecules. Pto harbors many PAMPs; thus for experimental ease, many studies utilize single synthesized PAMPs such as flg22, a short protein peptide derived from Pseudomonas flagellin. Flg22 recognition by Arabidopsis Flagellin Sensing 2 (FLS2) initiates a plethora of signaling responses including rapid production of apoplastic reactive oxygen species (ROS). Assessing flg22-ROS has been instrumental in identifying novel PAMP-signaling components; but comparably little is known whether in Arabidopsis, ROS is produced in response to intact live Pto and whether this response can be used to dissect genetic requirements of the plant host and live bacterial pathogens in planta. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 3 | 50% |
Nigeria | 1 | 17% |
Unknown | 2 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 67% |
Scientists | 1 | 17% |
Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 1 | <1% |
France | 1 | <1% |
Israel | 1 | <1% |
United Kingdom | 1 | <1% |
Mexico | 1 | <1% |
Belgium | 1 | <1% |
United States | 1 | <1% |
Unknown | 271 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 79 | 28% |
Researcher | 61 | 22% |
Student > Master | 31 | 11% |
Student > Doctoral Student | 23 | 8% |
Student > Bachelor | 22 | 8% |
Other | 29 | 10% |
Unknown | 33 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 168 | 60% |
Biochemistry, Genetics and Molecular Biology | 54 | 19% |
Chemistry | 5 | 2% |
Medicine and Dentistry | 4 | 1% |
Nursing and Health Professions | 2 | <1% |
Other | 12 | 4% |
Unknown | 33 | 12% |