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Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros

Overview of attention for article published in Journal of Venomous Animals and Toxins including Tropical Diseases, June 2017
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  • Above-average Attention Score compared to outputs of the same age (54th percentile)
  • Good Attention Score compared to outputs of the same age and source (77th percentile)

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2 Facebook pages

Citations

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Title
Isolation and biochemical characterization of bradykinin-potentiating peptides from Bitis gabonica rhinoceros
Published in
Journal of Venomous Animals and Toxins including Tropical Diseases, June 2017
DOI 10.1186/s40409-017-0124-9
Pubmed ID
Authors

Tamara M. Fucase, Juliana M. Sciani, Ingrid Cavalcante, Vincent L. Viala, Bruno B. Chagas, Daniel C. Pimenta, Patrick J. Spencer

Abstract

Venoms represent a still underexplored reservoir of bioactive components that might mitigate or cure diseases in conditions in which conventional therapy is ineffective. The bradykinin-potentiating peptides (BPPs) comprise a class of angiotensin-I converting enzyme (ACE) inhibitors. The BPPs usually consist of oligopeptides with 5 to 13 residues with a high number of proline residues and the tripeptide Ile-Pro-Pro (IPP-tripeptide) in the C-terminus region and have a conserved N-terminal pyroglutamate residue. As a whole, the action of the BPPs on prey and snakebite victims results in the decrease of the blood pressure. The aim of this work was to isolate and characterize novel BPPs from the venom of Bitis gabonica rhinoceros. The crude venom of B. g. rhinoceros was fractionated by size exclusion chromatography and the peptide fraction (<7 kDa) was separated by reverse phase chromatography (RP-HPLC) and analyzed by ESI-IT-TOF-MS/MS. One new BPP was identified, synthetized and assayed for ACE inhibition and, in vivo, for edema potentiation. Typical BPP signatures were identified in three RP-HPLC fractions. CID fragmentation presented the usual y-ion of the terminal P-P fragment as a predominant signal at m/z 213.1. De novo peptide sequencing identified one Bothrops-like BPP and one new BPP sequence. The new BPP was synthesized and showed poor inhibition over ACE, but displayed significant bradykinin-induced edema potentiation. So far, few BPPs are described in Viperinae, and based on the sequenced peptides, two non-canonical sequences were detected. The possible clinical role of this new peptides remains unclear.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 18 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 39%
Student > Master 5 28%
Student > Bachelor 2 11%
Professor 1 6%
Student > Ph. D. Student 1 6%
Other 2 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 39%
Agricultural and Biological Sciences 4 22%
Immunology and Microbiology 1 6%
Arts and Humanities 1 6%
Earth and Planetary Sciences 1 6%
Other 2 11%
Unknown 2 11%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 July 2017.
All research outputs
#6,297,652
of 11,424,220 outputs
Outputs from Journal of Venomous Animals and Toxins including Tropical Diseases
#99
of 298 outputs
Outputs of similar age
#116,023
of 260,578 outputs
Outputs of similar age from Journal of Venomous Animals and Toxins including Tropical Diseases
#2
of 9 outputs
Altmetric has tracked 11,424,220 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 298 research outputs from this source. They receive a mean Attention Score of 3.1. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 260,578 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 7 of them.