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Cathepsin B inhibitor improves developmental competency and cryo-tolerance of in vitro ovine embryos

Overview of attention for article published in BMC Developmental Biology, July 2017
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Title
Cathepsin B inhibitor improves developmental competency and cryo-tolerance of in vitro ovine embryos
Published in
BMC Developmental Biology, July 2017
DOI 10.1186/s12861-017-0152-2
Pubmed ID
Authors

M. Pezhman, S. M. Hosseini, S. Ostadhosseini, Sh. Rouhollahi Varnosfaderani, F. Sefid, M. H. Nasr-Esfahani

Abstract

Cathepsin B is a lysosomal cysteine protease involved in apoptosis and oocytes which have lower developmental competence show higher expression of Cathepsin B. Furthermore, expression of Cathepsin B show a decreasing trend from oocyte toward blastocyst stage. Present study assessed the effect of cathepsin B inhibitor, E-64, on developmental competency and cryo-survival of pre-implantation ovine IVF derived embryos. Cathepsin B inhibitor was added during day 3 to 8 of development. One μM E-64 was defined as the optimal concentration required for improving blastocyst rate. This concentration also reduced DNA fragmentation and BAX as apoptotic markers while increasing total cell number per blastocyst and improving anti-apoptotic marker, the BCL2. We further showed that addition of 1.0 μM of E-64 during day 3 to 8 of development improved re-expansion and hatching rates of blastocysts post vitrification. E-64 also reduced rate of DNA fragmentation and BAX expression and increased total cell number per blastocyst and BCL2 expression post vitrification. However, addition of E-64 post vitrification reduced the hatching rate. Therefore, it can be concluded that inhibition of cathepsin B in IVC, not only improves quality and quantity of blastocysts but also improves the cryo-survival of in vitro derived blastocysts.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 25 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 16%
Student > Ph. D. Student 4 16%
Student > Doctoral Student 3 12%
Student > Bachelor 2 8%
Researcher 2 8%
Other 3 12%
Unknown 7 28%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 24%
Biochemistry, Genetics and Molecular Biology 5 20%
Chemistry 2 8%
Nursing and Health Professions 1 4%
Veterinary Science and Veterinary Medicine 1 4%
Other 2 8%
Unknown 8 32%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 July 2017.
All research outputs
#20,431,953
of 22,985,065 outputs
Outputs from BMC Developmental Biology
#336
of 371 outputs
Outputs of similar age
#273,455
of 313,617 outputs
Outputs of similar age from BMC Developmental Biology
#3
of 5 outputs
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So far Altmetric has tracked 371 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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