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The effects of probiotic Bacillus subtilis on the cytotoxicity of Clostridium perfringens type a in Caco-2 cell culture

Overview of attention for article published in BMC Microbiology, July 2017
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Title
The effects of probiotic Bacillus subtilis on the cytotoxicity of Clostridium perfringens type a in Caco-2 cell culture
Published in
BMC Microbiology, July 2017
DOI 10.1186/s12866-017-1051-1
Pubmed ID
Authors

Maryam Poormontaseri, Saeid Hosseinzadeh, Seyed Shahram Shekarforoush, Tahereh Kalantari

Abstract

Some Bacillus strains have recently been identified for potential use as probiotics and food additives. The present study evaluated the antimicrobial effects of Bacillus subtilis ATCC 6633 and its metabolite on the enterotoxin and vegetative cells, spore and germinated spore of Clostridium perfringens type A in Caco-2 cells. We used flow cytometry and MTT assays to evaluate the cytotoxicity effect of treatments. According to the results, the most cell survival was found in the 4% crude antimicrobial substance (CAS) with the vegetative form of C. perfringens among co-cultured groups. Furthermore, the apoptosis and necrosis in co-cultured groups were significantly decreased (P < 0.05). The present results suggested the crucial role of the current probiotic in the control of various forms of C. perfringens type A which was investigated for the first time. Also, the majority of treatments showed higher cell viability in flow cytometry compared to the MTT assay.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 54 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 17%
Student > Master 8 15%
Researcher 7 13%
Student > Bachelor 6 11%
Other 4 7%
Other 5 9%
Unknown 15 28%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 35%
Biochemistry, Genetics and Molecular Biology 6 11%
Veterinary Science and Veterinary Medicine 2 4%
Immunology and Microbiology 2 4%
Medicine and Dentistry 2 4%
Other 3 6%
Unknown 20 37%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 July 2017.
All research outputs
#20,431,953
of 22,985,065 outputs
Outputs from BMC Microbiology
#2,700
of 3,206 outputs
Outputs of similar age
#273,455
of 313,617 outputs
Outputs of similar age from BMC Microbiology
#47
of 58 outputs
Altmetric has tracked 22,985,065 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,206 research outputs from this source. They receive a mean Attention Score of 4.1. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 313,617 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.