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Physiological roles of sigma factor SigD in Corynebacterium glutamicum

Overview of attention for article published in BMC Microbiology, July 2017
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Title
Physiological roles of sigma factor SigD in Corynebacterium glutamicum
Published in
BMC Microbiology, July 2017
DOI 10.1186/s12866-017-1067-6
Pubmed ID
Authors

Hironori Taniguchi, Tobias Busche, Thomas Patschkowski, Karsten Niehaus, Miroslav Pátek, Jörn Kalinowski, Volker F. Wendisch

Abstract

Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail; however, the role of SigD in transcriptional regulation in C. glutamicum has been mostly unknown. In this work, pleiotropic effects of sigD overexpression at the level of phenotype, transcripts, proteins and metabolites were investigated. Overexpression of sigD decreased the growth rate of C. glutamicum cultures, and induced several physiological effects such as reduced culture foaming, turbid supernatant and cell aggregation. Upon overexpression of sigD, the level of Cmt1 (corynomycolyl transferase) in the supernatant was notably enhanced, and carbohydrate-containing compounds were excreted to the supernatant. The real-time PCR analysis revealed that sigD overexpression increased the expression of genes related to corynomycolic acid synthesis (fadD2, pks), genes encoding corynomycolyl transferases (cop1, cmt1, cmt2, cmt3), L, D-transpeptidase (lppS), a subunit of the major cell wall channel (porH), and the envelope lipid regulation factor (elrF). Furthermore, overexpression of sigD resulted in trehalose dicorynomycolate accumulation in the cell envelope. This study demonstrated that SigD regulates the synthesis of corynomycolate and related compounds, and expanded the knowledge of regulatory functions of sigma factors in C. glutamicum.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 18%
Student > Bachelor 4 18%
Professor 4 18%
Student > Master 4 18%
Other 1 5%
Other 2 9%
Unknown 3 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 9 41%
Agricultural and Biological Sciences 7 32%
Immunology and Microbiology 1 5%
Medicine and Dentistry 1 5%
Unknown 4 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 July 2017.
All research outputs
#10,172,552
of 11,465,445 outputs
Outputs from BMC Microbiology
#1,333
of 1,619 outputs
Outputs of similar age
#217,165
of 258,263 outputs
Outputs of similar age from BMC Microbiology
#37
of 48 outputs
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