Title |
Transcriptome analysis identifies Bacillus anthracis genes that respond to CO2through an AtxA-dependent mechanism
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Published in |
BMC Genomics, March 2014
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DOI | 10.1186/1471-2164-15-229 |
Pubmed ID | |
Authors |
Andrew T McKenzie, Andrei P Pomerantsev, Inka Sastalla, Craig Martens, Stacy M Ricklefs, Kimmo Virtaneva, Sarah Anzick, Stephen F Porcella, Stephen H Leppla |
Abstract |
Upon infection of a mammalian host, Bacillus anthracis responds to host cues, and particularly to elevated temperature (37°C) and bicarbonate/CO2 concentrations, with increased expression of virulence factors that include the anthrax toxins and extracellular capsular layer. This response requires the presence of the pXO1 virulence plasmid-encoded pleiotropic regulator AtxA. To better understand the genetic basis of this response, we utilized a controlled in vitro system and Next Generation sequencing to determine and compare RNA expression profiles of the parental strain and an isogenic AtxA-deficient strain in a 2 × 2 factorial design with growth environments containing or lacking carbon dioxide. |
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Netherlands | 1 | 25% |
France | 1 | 25% |
United Kingdom | 1 | 25% |
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Scientists | 4 | 100% |
Mendeley readers
Geographical breakdown
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South Africa | 1 | 3% |
Brazil | 1 | 3% |
Unknown | 37 | 95% |
Demographic breakdown
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Researcher | 12 | 31% |
Student > Ph. D. Student | 7 | 18% |
Student > Doctoral Student | 5 | 13% |
Student > Master | 4 | 10% |
Professor > Associate Professor | 3 | 8% |
Other | 6 | 15% |
Unknown | 2 | 5% |
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Computer Science | 2 | 5% |
Other | 3 | 8% |
Unknown | 3 | 8% |