Title |
Validation of the ligase detection reaction fluorescent microsphere assay for the detection of Plasmodium falciparum resistance mediating polymorphisms in Uganda
|
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Published in |
Malaria Journal, March 2014
|
DOI | 10.1186/1475-2875-13-95 |
Pubmed ID | |
Authors |
Sheila Nankoberanyi, George W Mbogo, Norbert P LeClair, Melissa D Conrad, Patrick Tumwebaze, Stephen Tukwasibwe, Moses R Kamya, Jordan Tappero, Samuel L Nsobya, Philip J Rosenthal |
Abstract |
Malaria remains a major public health problem, and its control has been hampered by drug resistance. For a number of drugs, Plasmodium falciparum single nucleotide polymorphisms (SNPs) are associated with altered drug sensitivity and can be used as markers of drug resistance. Several techniques have been studied to assess resistance markers. The most widely used methodology is restriction fragment length polymorphism (RFLP) analysis. The ligase detection reaction fluorescent microsphere (LDR-FM) assay was recently shown to provide high throughput assessment of P. falciparum SNPs associated with drug resistance. The aim of this study was to validate the reliability and accuracy of the LDR-FM assay in a field setting. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 2 | 3% |
Burkina Faso | 1 | 2% |
Unknown | 63 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 14 | 21% |
Student > Master | 10 | 15% |
Researcher | 9 | 14% |
Student > Bachelor | 5 | 8% |
Student > Doctoral Student | 4 | 6% |
Other | 14 | 21% |
Unknown | 10 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 13 | 20% |
Agricultural and Biological Sciences | 13 | 20% |
Biochemistry, Genetics and Molecular Biology | 7 | 11% |
Computer Science | 4 | 6% |
Social Sciences | 4 | 6% |
Other | 14 | 21% |
Unknown | 11 | 17% |