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Toll-like receptor 2-mediated NF-kappa B pathway activation in ocular surface epithelial cells

Overview of attention for article published in Eye and Vision, July 2017
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Title
Toll-like receptor 2-mediated NF-kappa B pathway activation in ocular surface epithelial cells
Published in
Eye and Vision, July 2017
DOI 10.1186/s40662-017-0082-x
Pubmed ID
Authors

Aihua Hou, Min Qi Tin, Louis Tong, Aihua Hou, Min Qi Tin, Louis Tong

Abstract

Gram-positive bacteria stimulate Toll-like receptor (TLR) 2 and then activate the pro-inflammatory nuclear factor-kappa B (NF-κB) pathway. As the human ocular surface is heavily colonised by gram-positive cocci bacteria, a balance of activation/repression of NF-κB target genes is essential to avoid uncontrolled infection or autoimmune-related inflammation. It is advantageous to test NF-κB targeting molecules in an ocular surface culture system that allows assessment of temporal NF-κB activation in a longitudinal fashion without destruction of cells. Such initial testing under standardised conditions should reduce the number of molecules that progress to further evaluation in animal models. This study aims to establish an in-vitro cell culture system to assess NF-κB activation in the context of ocular surface cells. NF-κB activity was evaluated through a secretory alkaline phosphatase reporter assay (SEAP). Immunoblots and immunofluorescence were used to examine IκBα phosphorylation and p65/p50 nuclear localization. Monocyte chemoattractant protein-1 (MCP-1) transcripts were evaluated by real time PCR and protein levels were measured by ELISA. NF-κB activity in HCE-T cells treated with TLR2 activator Pam3CSK4 was higher than control cells at both 6 and 24 h. Pam3CSK4-stimulated NF-κB activation was inhibited by IκK inhibitors, Wedelolactone and BMS-345541. In Pam3CSK4 treated cells, active NF-κB subunits p50 and p65 increased in cell nuclear fractions as early as 1.5 h. Although the level of total IκB-α remained constant, phospho-IκB-α increased with treatment over time. In the culture media of Pam3CSK4-stimulated cells, MCP-1 protein level was increased, which was suppressed in the presence of IκK inhibitors. NF-κB pathway can be activated by the TLR2 ligand and inhibited by IκK inhibitors in the ocular surface cell culture system. This cell culture system may be used to evaluate TLR-related innate defences in ocular surface diseases.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 24%
Student > Doctoral Student 2 12%
Student > Ph. D. Student 2 12%
Student > Bachelor 2 12%
Researcher 1 6%
Other 1 6%
Unknown 5 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 24%
Agricultural and Biological Sciences 3 18%
Immunology and Microbiology 2 12%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Chemical Engineering 1 6%
Other 1 6%
Unknown 5 29%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 October 2017.
All research outputs
#19,085,470
of 21,446,675 outputs
Outputs from Eye and Vision
#103
of 214 outputs
Outputs of similar age
#247,206
of 285,391 outputs
Outputs of similar age from Eye and Vision
#1
of 5 outputs
Altmetric has tracked 21,446,675 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 214 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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