Title |
Serum-free microcarrier based production of replication deficient Influenza vaccine candidate virus lacking NS1 using Vero cells
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Published in |
BMC Biotechnology, August 2011
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DOI | 10.1186/1472-6750-11-81 |
Pubmed ID | |
Authors |
Allen Chen, Swan Li Poh, Christian Dietzsch, Elisabeth Roethl, Mylene L Yan, Say Kong Ng |
Abstract |
Influenza virus is a major health concern that has huge impacts on the human society, and vaccination remains as one of the most effective ways to mitigate this disease. Comparing the two types of commercially available Influenza vaccine, the live attenuated virus vaccine is more cross-reactive and easier to administer than the traditional inactivated vaccines. One promising live attenuated Influenza vaccine that has completed Phase I clinical trial is deltaFLU, a deletion mutant lacking the viral Nonstructural Protein 1 (NS1) gene. As a consequence of this gene deletion, this mutant virus can only propagate effectively in cells with a deficient interferon-mediated antiviral response. To demonstrate the manufacturability of this vaccine candidate, a batch bioreactor production process using adherent Vero cells on microcarriers in commercially available animal-component free, serum-free media is described. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Malaysia | 1 | 1% |
Unknown | 70 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 22 | 31% |
Student > Master | 10 | 14% |
Student > Ph. D. Student | 9 | 13% |
Student > Bachelor | 8 | 11% |
Student > Doctoral Student | 5 | 7% |
Other | 4 | 6% |
Unknown | 13 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 17 | 24% |
Engineering | 9 | 13% |
Biochemistry, Genetics and Molecular Biology | 6 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 7% |
Medicine and Dentistry | 5 | 7% |
Other | 13 | 18% |
Unknown | 16 | 23% |