Title |
Model SNP development for complex genomes based on hexaploid oat using high-throughput 454 sequencing technology
|
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Published in |
BMC Genomics, January 2011
|
DOI | 10.1186/1471-2164-12-77 |
Pubmed ID | |
Authors |
Rebekah E Oliver, Gerard R Lazo, Joseph D Lutz, Marc J Rubenfield, Nicholas A Tinker, Joseph M Anderson, Nicole H Wisniewski Morehead, Dinesh Adhikary, Eric N Jellen, P Jeffrey Maughan, Gina L Brown Guedira, Shiaoman Chao, Aaron D Beattie, Martin L Carson, Howard W Rines, Donald E Obert, J Michael Bonman, Eric W Jackson |
Abstract |
Genetic markers are pivotal to modern genomics research; however, discovery and genotyping of molecular markers in oat has been hindered by the size and complexity of the genome, and by a scarcity of sequence data. The purpose of this study was to generate oat expressed sequence tag (EST) information, develop a bioinformatics pipeline for SNP discovery, and establish a method for rapid, cost-effective, and straightforward genotyping of SNP markers in complex polyploid genomes such as oat. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Italy | 2 | 1% |
Netherlands | 1 | <1% |
Pakistan | 1 | <1% |
Norway | 1 | <1% |
Brazil | 1 | <1% |
Sweden | 1 | <1% |
Canada | 1 | <1% |
Slovenia | 1 | <1% |
Argentina | 1 | <1% |
Other | 2 | 1% |
Unknown | 127 | 91% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 37 | 27% |
Student > Ph. D. Student | 27 | 19% |
Student > Master | 13 | 9% |
Professor > Associate Professor | 9 | 6% |
Student > Doctoral Student | 8 | 6% |
Other | 23 | 17% |
Unknown | 22 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 94 | 68% |
Biochemistry, Genetics and Molecular Biology | 9 | 6% |
Environmental Science | 3 | 2% |
Computer Science | 2 | 1% |
Medicine and Dentistry | 2 | 1% |
Other | 3 | 2% |
Unknown | 26 | 19% |