Title |
Expression profiling of formalin-fixed paraffin-embedded primary breast tumors using cancer-specific and whole genome gene panels on the DASL®platform
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Published in |
BMC Medical Genomics, December 2010
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DOI | 10.1186/1755-8794-3-60 |
Pubmed ID | |
Authors |
Monica M Reinholz, Jeanette E Eckel-Passow, S Keith Anderson, Yan W Asmann, Michael A Zschunke, Ann L Oberg, Ann E McCullough, Amylou C Dueck, Beiyun Chen, Craig S April, Eliza Wickham-Garcia, Robert B Jenkins, Julie M Cunningham, Jin Jen, Edith A Perez, Jian-Bing Fan, Wilma L Lingle |
Abstract |
The cDNA-mediated Annealing, extension, Selection and Ligation (DASL) assay has become a suitable gene expression profiling system for degraded RNA from paraffin-embedded tissue. We examined assay characteristics and the performance of the DASL 502-gene Cancer Panel v1 (1.5K) and 24,526-gene panel (24K) platforms at differentiating nine human epidermal growth factor receptor 2- positive (HER2+) and 11 HER2-negative (HER2-) paraffin-embedded breast tumors. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Netherlands | 1 | 3% |
Unknown | 29 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 9 | 30% |
Student > Ph. D. Student | 5 | 17% |
Student > Master | 4 | 13% |
Other | 3 | 10% |
Student > Postgraduate | 2 | 7% |
Other | 6 | 20% |
Unknown | 1 | 3% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 10 | 33% |
Agricultural and Biological Sciences | 9 | 30% |
Biochemistry, Genetics and Molecular Biology | 4 | 13% |
Immunology and Microbiology | 1 | 3% |
Physics and Astronomy | 1 | 3% |
Other | 1 | 3% |
Unknown | 4 | 13% |