Expression profiling of formalin-fixed paraffin-embedded primary breast tumors using cancer-specific and whole genome gene panels on the DASL® platform.
BMC Medical Genomics, December 2010
Reinholz MM, Eckel-Passow JE, Anderson SK, Asmann YW, Zschunke MA, Oberg AL, McCullough AE, Dueck AC, Chen B, April CS, Wickham-Garcia E, Jenkins RB, Cunningham JM, Jen J, Perez EA, Fan JB, Lingle WL
The cDNA-mediated Annealing, extension, Selection and Ligation (DASL) assay has become a suitable gene expression profiling system for degraded RNA from paraffin-embedded tissue. We examined assay characteristics and the performance of the DASL 502-gene Cancer Panel v1 (1.5K) and 24,526-gene panel (24K) platforms at differentiating nine human epidermal growth factor receptor 2- positive (HER2+) and 11 HER2-negative (HER2-) paraffin-embedded breast tumors.
|Readers by professional status||Count||As %|
|Student > Ph. D. Student||5||17%|
|Student > Master||5||17%|
|Student > Postgraduate||2||7%|
|Readers by discipline||Count||As %|
|Medicine and Dentistry||10||33%|
|Agricultural and Biological Sciences||10||33%|
|Biochemistry, Genetics and Molecular Biology||4||13%|
|Immunology and Microbiology||1||3%|
|Physics and Astronomy||1||3%|