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Capped antigenomic RNA transcript facilitates rescue of a plant rhabdovirus

Overview of attention for article published in Virology Journal, June 2017
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Title
Capped antigenomic RNA transcript facilitates rescue of a plant rhabdovirus
Published in
Virology Journal, June 2017
DOI 10.1186/s12985-017-0776-7
Pubmed ID
Authors

Shasha Qian, Xiaolan Chen, Kai Sun, Yang Zhang, Zhenghe Li

Abstract

Recovery of recombinant negative-stranded RNA viruses from cloned cDNAs is an inefficient process as multiple viral components need to be delivered into cells for reconstitution of infectious entities. Previously studies have shown that authentic viral RNA termini are essential for efficient virus rescue. However, little is known about the activity of viral RNAs processed by different strategies in supporting recovery of plant negative-stranded RNA virus. In this study, we used several versions of hammerhead ribozymes and a truncated cauliflower mosaic virus 35S promoter to generate precise 5' termini of sonchus yellow net rhabdovirus (SYNV) antigenomic RNA (agRNA) derivatives. These agRNAs were co-expressed with the SYNV core proteins in Nicotiana benthamiana leaves to evaluate their efficiency in supporting fluorescent reporter gene expression from an SYNV minireplicon (MR) and rescue of full-length virus. Optimization of hammerhead ribozyme cleavage activities led to improved SYNV MR reporter gene expression. Although the MR agRNA processed by the most active hammerhead variants is comparable to the capped, precisely transcribed agRNA in supporting MR activity, efficient recovery of recombinant SYNV was only achieved with capped agRNA. Further studies showed that the capped SYNV agRNA permitted transient expression of the nucleocapsid (N) protein, and an agRNA derivatives unable to express the N protein in cis exhibited dramatically reduced rescue efficiency. Our study reveals superior activity of precisely transcribed, capped SYNV agRNAs to uncapped, hammerhead ribozyme-processed agRNAs, and suggests a cis-acting function for the N protein expressed from the capped agRNA during recovery of SYNV from plasmids.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 36%
Professor 2 18%
Professor > Associate Professor 2 18%
Student > Ph. D. Student 1 9%
Unknown 2 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 55%
Biochemistry, Genetics and Molecular Biology 2 18%
Unknown 3 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 March 2018.
All research outputs
#10,503,829
of 13,789,144 outputs
Outputs from Virology Journal
#1,579
of 2,217 outputs
Outputs of similar age
#180,207
of 265,673 outputs
Outputs of similar age from Virology Journal
#1
of 1 outputs
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