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Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species

Overview of attention for article published in BMC Genomics, April 2008
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Title
Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species
Published in
BMC Genomics, April 2008
DOI 10.1186/1471-2164-9-167
Pubmed ID
Authors

Nicolas González, Stephan Heeb, Claudio Valverde, Elisabeth Kay, Cornelia Reimmann, Thomas Junier, Dieter Haas

Abstract

Small RNAs (sRNAs) are widespread among bacteria and have diverse regulatory roles. Most of these sRNAs have been discovered by a combination of computational and experimental methods. In Pseudomonas aeruginosa, a ubiquitous Gram-negative bacterium and opportunistic human pathogen, the GacS/GacA two-component system positively controls the transcription of two sRNAs (RsmY, RsmZ), which are crucial for the expression of genes involved in virulence. In the biocontrol bacterium Pseudomonas fluorescens CHA0, three GacA-controlled sRNAs (RsmX, RsmY, RsmZ) regulate the response to oxidative stress and the expression of extracellular products including biocontrol factors. RsmX, RsmY and RsmZ contain multiple unpaired GGA motifs and control the expression of target mRNAs at the translational level, by sequestration of translational repressor proteins of the RsmA family. A combined computational and experimental approach enabled us to identify 14 intergenic regions encoding sRNAs in P. aeruginosa. Eight of these regions encode newly identified sRNAs. The intergenic region 1698 was found to specify a novel GacA-controlled sRNA termed RgsA. GacA regulation appeared to be indirect. In P. fluorescens CHA0, an RgsA homolog was also expressed under positive GacA control. This 120-nt sRNA contained a single GGA motif and, unlike RsmX, RsmY and RsmZ, was unable to derepress translation of the hcnA gene (involved in the biosynthesis of the biocontrol factor hydrogen cyanide), but contributed to the bacterium's resistance to hydrogen peroxide. In both P. aeruginosa and P. fluorescens the stress sigma factor RpoS was essential for RgsA expression. The discovery of an additional sRNA expressed under GacA control in two Pseudomonas species highlights the complexity of this global regulatory system and suggests that the mode of action of GacA control may be more elaborate than previously suspected. Our results also confirm that several GGA motifs are required in an sRNA for sequestration of the RsmA protein.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 117 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 3%
United Kingdom 1 <1%
Slovenia 1 <1%
Iran, Islamic Republic of 1 <1%
Denmark 1 <1%
Belgium 1 <1%
Unknown 108 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 35 30%
Student > Ph. D. Student 30 26%
Student > Master 12 10%
Student > Doctoral Student 7 6%
Student > Bachelor 6 5%
Other 18 15%
Unknown 9 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 68 58%
Biochemistry, Genetics and Molecular Biology 22 19%
Immunology and Microbiology 2 2%
Environmental Science 2 2%
Chemistry 2 2%
Other 9 8%
Unknown 12 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 July 2017.
All research outputs
#7,534,941
of 22,990,068 outputs
Outputs from BMC Genomics
#3,631
of 10,691 outputs
Outputs of similar age
#28,541
of 81,895 outputs
Outputs of similar age from BMC Genomics
#9
of 28 outputs
Altmetric has tracked 22,990,068 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 10,691 research outputs from this source. They receive a mean Attention Score of 4.7. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 81,895 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 18th percentile – i.e., 18% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.