Title |
MPprimer: a program for reliable multiplex PCR primer design
|
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Published in |
BMC Bioinformatics, March 2010
|
DOI | 10.1186/1471-2105-11-143 |
Pubmed ID | |
Authors |
Zhiyong Shen, Wubin Qu, Wen Wang, Yiming Lu, Yonghong Wu, Zhifeng Li, Xingyi Hang, Xiaolei Wang, Dongsheng Zhao, Chenggang Zhang |
Abstract |
Multiplex PCR, defined as the simultaneous amplification of multiple regions of a DNA template or multiple DNA templates using more than one primer set (comprising a forward primer and a reverse primer) in one tube, has been widely used in diagnostic applications of clinical and environmental microbiology studies. However, primer design for multiplex PCR is still a challenging problem and several factors need to be considered. These problems include mis-priming due to nonspecific binding to non-target DNA templates, primer dimerization, and the inability to separate and purify DNA amplicons with similar electrophoretic mobility. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 7 | 2% |
France | 5 | 1% |
United Kingdom | 4 | 1% |
China | 2 | <1% |
Belgium | 2 | <1% |
Brazil | 2 | <1% |
Armenia | 1 | <1% |
Italy | 1 | <1% |
Sweden | 1 | <1% |
Other | 10 | 3% |
Unknown | 328 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 85 | 23% |
Student > Ph. D. Student | 61 | 17% |
Student > Master | 55 | 15% |
Student > Bachelor | 30 | 8% |
Student > Postgraduate | 16 | 4% |
Other | 64 | 18% |
Unknown | 52 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 157 | 43% |
Biochemistry, Genetics and Molecular Biology | 78 | 21% |
Computer Science | 14 | 4% |
Medicine and Dentistry | 13 | 4% |
Immunology and Microbiology | 8 | 2% |
Other | 36 | 10% |
Unknown | 57 | 16% |