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A novel, non-radioactive eukaryotic in vitro transcription assay for sensitive quantification of RNA polymerase II activity

Overview of attention for article published in BMC Molecular Biology, January 2014
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Mentioned by

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2 tweeters

Citations

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11 Dimensions

Readers on

mendeley
53 Mendeley
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1 CiteULike
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Title
A novel, non-radioactive eukaryotic in vitro transcription assay for sensitive quantification of RNA polymerase II activity
Published in
BMC Molecular Biology, January 2014
DOI 10.1186/1471-2199-15-7
Pubmed ID
Authors

Cristina Voss, Brita Schmitt, Susanne Werner-Simon, Christian Lutz, Werner Simon, Jan Anderl

Abstract

Many studies of the eukaryotic transcription mechanism and its regulation rely on in vitro assays. Conventional RNA polymerase II transcription assays are based on radioactive labelling of the newly synthesized RNA. Due to the inefficient in vitro transcription, the detection of the RNA involving purification and gel electrophoresis is laborious and not always quantitative.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
Germany 1 2%
Unknown 51 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 32%
Researcher 11 21%
Student > Doctoral Student 5 9%
Student > Master 4 8%
Other 3 6%
Other 7 13%
Unknown 6 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 17 32%
Agricultural and Biological Sciences 16 30%
Chemistry 5 9%
Medicine and Dentistry 3 6%
Pharmacology, Toxicology and Pharmaceutical Science 2 4%
Other 3 6%
Unknown 7 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 April 2014.
All research outputs
#9,479,879
of 12,378,087 outputs
Outputs from BMC Molecular Biology
#168
of 247 outputs
Outputs of similar age
#123,573
of 197,836 outputs
Outputs of similar age from BMC Molecular Biology
#1
of 2 outputs
Altmetric has tracked 12,378,087 research outputs across all sources so far. This one is in the 20th percentile – i.e., 20% of other outputs scored the same or lower than it.
So far Altmetric has tracked 247 research outputs from this source. They receive a mean Attention Score of 2.7. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 197,836 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them