Title |
A novel molecular typing method of Mycobacteria based on DNA barcoding visualization
|
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Published in |
Journal of Clinical Bioinformatics, February 2014
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DOI | 10.1186/2043-9113-4-4 |
Pubmed ID | |
Authors |
Bin Liu, Xiaotian Zhang, Honglan Huang, Ying Zhang, Fengfeng Zhou, Guoqing Wang |
Abstract |
Different subtypes of Mycobacterium tuberculosis (MTB) may induce diverse severe human infections, and some of their symptoms are similar to other pathogenes, e.g. Nontuberculosis mycobacteria (NTM). So determination of mycobacterium subtypes facilitates the effective control of MTB infection and proliferation. This study exploits a novel DNA barcoding visualization method for molecular typing of 17 mycobacteria genomes published in the NCBI prokaryotic genome database. Three mycobacterium genes (Rv0279c, Rv3508 and Rv3514) from the PE/PPE family of MT Band were detected to best represent the inter-strain pathogenetic variations. An accurate and fast MTB substrain typing method was proposed based on the combination of the aforementioned three biomarker genes and the 16S rRNA gene. The protocol of establishing a bacterial substrain typing system used in this study may also be applied to the other pathogenes. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Brazil | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Colombia | 1 | 5% |
Unknown | 18 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 6 | 32% |
Researcher | 5 | 26% |
Lecturer > Senior Lecturer | 2 | 11% |
Student > Ph. D. Student | 1 | 5% |
Other | 1 | 5% |
Other | 2 | 11% |
Unknown | 2 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 32% |
Biochemistry, Genetics and Molecular Biology | 5 | 26% |
Medicine and Dentistry | 3 | 16% |
Immunology and Microbiology | 2 | 11% |
Computer Science | 1 | 5% |
Other | 0 | 0% |
Unknown | 2 | 11% |