Title |
Innate immune response to a H3N2 subtype swine influenza virus in newborn porcine trachea cells, alveolar macrophages, and precision-cut lung slices
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Published in |
Veterinary Research, April 2014
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DOI | 10.1186/1297-9716-45-42 |
Pubmed ID | |
Authors |
Mario Delgado-Ortega, Sandrine Melo, Darsaniya Punyadarsaniya, Christelle Ramé, Michel Olivier, Denis Soubieux, Daniel Marc, Gaëlle Simon, Georg Herrler, Mustapha Berri, Joëlle Dupont, François Meurens |
Abstract |
Viral respiratory diseases remain of major importance in swine breeding units. Swine influenza virus (SIV) is one of the main known contributors to infectious respiratory diseases. The innate immune response to swine influenza viruses has been assessed in many previous studies. However most of these studies were carried out in a single-cell population or directly in the live animal, in all its complexity. In the current study we report the use of a trachea epithelial cell line (newborn pig trachea cells - NPTr) in comparison with alveolar macrophages and lung slices for the characterization of innate immune response to an infection by a European SIV of the H3N2 subtype. The expression pattern of transcripts involved in the recognition of the virus, interferon type I and III responses, and the host-response regulation were assessed by quantitative PCR in response to infection. Some significant differences were observed between the three systems, notably in the expression of type III interferon mRNA. Then, results show a clear induction of JAK/STAT and MAPK signaling pathways in infected NPTr cells. Conversely, PI3K/Akt signaling pathways was not activated. The inhibition of the JAK/STAT pathway clearly reduced interferon type I and III responses and the induction of SOCS1 at the transcript level in infected NPTr cells. Similarly, the inhibition of MAPK pathway reduced viral replication and interferon response. All together, these results contribute to an increased understanding of the innate immune response to H3N2 SIV and may help identify strategies to effectively control SIV infection. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 33% |
Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Members of the public | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Hungary | 1 | 1% |
Canada | 1 | 1% |
Unknown | 69 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 16 | 23% |
Student > Ph. D. Student | 16 | 23% |
Researcher | 13 | 18% |
Student > Bachelor | 5 | 7% |
Student > Doctoral Student | 4 | 6% |
Other | 8 | 11% |
Unknown | 9 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 19 | 27% |
Immunology and Microbiology | 12 | 17% |
Veterinary Science and Veterinary Medicine | 10 | 14% |
Biochemistry, Genetics and Molecular Biology | 5 | 7% |
Medicine and Dentistry | 5 | 7% |
Other | 9 | 13% |
Unknown | 11 | 15% |