Title |
Magnetic hyperthermia enhance the treatment efficacy of peri-implant osteomyelitis
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Published in |
BMC Infectious Diseases, July 2017
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DOI | 10.1186/s12879-017-2621-4 |
Pubmed ID | |
Authors |
Chih-Hsiang Fang, Pei-I Tsai, Shu-Wei Huang, Jui-Sheng Sun, Jenny Zwei-Chieng Chang, Hsin-Hsin Shen, San-Yuan Chen, Feng Huei Lin, Lih-Tao Hsu, Yen-Chun Chen |
Abstract |
When bacteria colony persist within a biofilm, suitable drugs are not yet available for the eradication of biofilm-producing bacteria. The aim of this study is to study the effect of magnetic nano-particles-induced hyperthermia on destroying biofilm and promoting bactericidal effects of antibiotics in the treatment of osteomyelitis. Sixty 12-weeks-old male Wistar rats were used. A metallic 18G needle was implanted into the bone marrow cavity of distal femur after the injection of Methicillin-sensitive Staphylococcus aureus (MSSA). All animals were divided into 5 different treatment modalities. The microbiological evaluation, scanning electron microscope examination, radiographic examination and then micro-CT evaluation of peri-implant bone resorption were analyzed. The pathomorphological characteristics of biofilm formation were completed after 40-days induction of osteomyelitis. The inserted implants can be heated upto 75 °C by magnetic heating without any significant thermal damage on the surrounding tissue. We also demonstrated that systemic administration of vancomycin [VC (i.m.)] could not eradicate the bacteria; but, local administration of vancomycin into the femoral canal and the presence of magnetic nanoparticles hyperthermia did enhance the eradication of bacteria in a biofilm-based colony. In these two groups, the percent bone volume (BV/TV: %) was significantly higher than that of the positive control. For the treatment of chronic osteomyelitis, we developed a new modality to improve antibiotic efficacy; the protection effect of biofilms on bacteria could be destroyed by magnetic nanoparticles-induced hyperthermia and therapeutic effect of systemic antibiotics could be enhanced. |
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Demographic breakdown
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
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Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 15 | 16% |
Student > Master | 13 | 14% |
Student > Bachelor | 11 | 12% |
Researcher | 8 | 9% |
Student > Doctoral Student | 3 | 3% |
Other | 11 | 12% |
Unknown | 30 | 33% |
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Engineering | 9 | 10% |
Materials Science | 8 | 9% |
Immunology and Microbiology | 4 | 4% |
Biochemistry, Genetics and Molecular Biology | 4 | 4% |
Other | 16 | 18% |
Unknown | 37 | 41% |