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Modulation of chromatin structure by the FACT histone chaperone complex regulates HIV-1 integration

Overview of attention for article published in Retrovirology, July 2017
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  • Above-average Attention Score compared to outputs of the same age (58th percentile)

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5 tweeters

Citations

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21 Dimensions

Readers on

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43 Mendeley
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Title
Modulation of chromatin structure by the FACT histone chaperone complex regulates HIV-1 integration
Published in
Retrovirology, July 2017
DOI 10.1186/s12977-017-0363-4
Pubmed ID
Authors

Julien Matysiak, Paul Lesbats, Eric Mauro, Delphine Lapaillerie, Jean-William Dupuy, Angelica P. Lopez, Mohamed Salah Benleulmi, Christina Calmels, Marie-Line Andreola, Marc Ruff, Manuel Llano, Olivier Delelis, Marc Lavigne, Vincent Parissi

Abstract

Insertion of retroviral genome DNA occurs in the chromatin of the host cell. This step is modulated by chromatin structure as nucleosomes compaction was shown to prevent HIV-1 integration and chromatin remodeling has been reported to affect integration efficiency. LEDGF/p75-mediated targeting of the integration complex toward RNA polymerase II (polII) transcribed regions ensures optimal access to dynamic regions that are suitable for integration. Consequently, we have investigated the involvement of polII-associated factors in the regulation of HIV-1 integration. Using a pull down approach coupled with mass spectrometry, we have selected the FACT (FAcilitates Chromatin Transcription) complex as a new potential cofactor of HIV-1 integration. FACT is a histone chaperone complex associated with the polII transcription machinery and recently shown to bind LEDGF/p75. We report here that a tripartite complex can be formed between HIV-1 integrase, LEDGF/p75 and FACT in vitro and in cells. Biochemical analyzes show that FACT-dependent nucleosome disassembly promotes HIV-1 integration into chromatinized templates, and generates highly favored nucleosomal structures in vitro. This effect was found to be amplified by LEDGF/p75. Promotion of this FACT-mediated chromatin remodeling in cells both increases chromatin accessibility and stimulates HIV-1 infectivity and integration. Altogether, our data indicate that FACT regulates HIV-1 integration by inducing local nucleosomes dissociation that modulates the functional association between the incoming intasome and the targeted nucleosome.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 43 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 13 30%
Student > Ph. D. Student 11 26%
Student > Postgraduate 5 12%
Student > Master 5 12%
Professor 2 5%
Other 1 2%
Unknown 6 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 14 33%
Agricultural and Biological Sciences 12 28%
Medicine and Dentistry 5 12%
Immunology and Microbiology 3 7%
Physics and Astronomy 1 2%
Other 0 0%
Unknown 8 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 September 2018.
All research outputs
#7,065,343
of 13,477,526 outputs
Outputs from Retrovirology
#379
of 799 outputs
Outputs of similar age
#109,177
of 265,634 outputs
Outputs of similar age from Retrovirology
#1
of 1 outputs
Altmetric has tracked 13,477,526 research outputs across all sources so far. This one is in the 47th percentile – i.e., 47% of other outputs scored the same or lower than it.
So far Altmetric has tracked 799 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.8. This one has gotten more attention than average, scoring higher than 50% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,634 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 58% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them