Title |
A genome scale overexpression screen to reveal drug activity in human cells
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Published in |
Genome Medicine, April 2014
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DOI | 10.1186/gm549 |
Pubmed ID | |
Authors |
Anthony Arnoldo, Saranya Kittanakom, Lawrence E Heisler, Anthony B Mak, Andrey I Shukalyuk, Dax Torti, Jason Moffat, Guri Giaever, Corey Nislow |
Abstract |
Target identification is a critical step in the lengthy and expensive process of drug development. Here, we describe a genome-wide screening platform that uses systematic overexpression of pooled human ORFs to understand drug mode-of-action and resistance mechanisms. We first calibrated our screen with the well-characterized drug methotrexate. We then identified new genes involved in the bioactivity of diverse drugs including antineoplastic agents and biologically active molecules. Finally, we focused on the transcription factor RHOXF2 whose overexpression conferred resistance to DNA damaging agents. This approach represents an orthogonal method for functional screening and, to our knowledge, has never been reported before. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 2% |
Italy | 1 | 2% |
Germany | 1 | 2% |
Unknown | 53 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 13 | 23% |
Researcher | 12 | 21% |
Student > Bachelor | 6 | 11% |
Student > Master | 6 | 11% |
Lecturer | 3 | 5% |
Other | 7 | 13% |
Unknown | 9 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 20 | 36% |
Agricultural and Biological Sciences | 13 | 23% |
Immunology and Microbiology | 4 | 7% |
Computer Science | 3 | 5% |
Neuroscience | 2 | 4% |
Other | 3 | 5% |
Unknown | 11 | 20% |