Title |
Butyrate induced changes in Wnt-signaling specific gene expression in colorectal cancer cells
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Published in |
BMC Research Notes, April 2014
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DOI | 10.1186/1756-0500-7-226 |
Pubmed ID | |
Authors |
Darina L Lazarova, Christopher Chiaro, Michael Bordonaro |
Abstract |
We have determined that butyrate, which is derived from the fermentation of dietary fiber in the colonic lumen, hyperactivates Wnt activity in colorectal (CRC) cells, and that this upregulation of Wnt signaling is causatively related to the induction of apoptosis. To better understand the genetic program regulated by butyrate-mediated Wnt hyperactivation, we performed total human genome microarray analyses on HCT-116 CRC cells in the presence or absence of a physiologically relevant concentration of butyrate. To evaluate changes in Wnt-specific gene expression, Wnt activity was suppressed with inducible dominant negative Tcf4 (DN-Tcf4). Six biological replicates of a full human genome microarray were performed, and the data deposited into the Gene Expression Omnibus database, according to Minimum Information About A Microarray Experiment standards. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Central African Republic | 1 | 50% |
Venezuela, Bolivarian Republic of | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 43 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 9 | 21% |
Student > Bachelor | 5 | 12% |
Student > Doctoral Student | 3 | 7% |
Researcher | 3 | 7% |
Student > Master | 3 | 7% |
Other | 7 | 16% |
Unknown | 13 | 30% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 7 | 16% |
Medicine and Dentistry | 4 | 9% |
Immunology and Microbiology | 2 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Other | 4 | 9% |
Unknown | 15 | 35% |