↓ Skip to main content

BaltDC: purification, characterization and infrared spectroscopy of an antiplatelet DC protein isolated from Bothrops alternatus snake venom

Overview of attention for article published in Journal of Venomous Animals and Toxins including Tropical Diseases, July 2017
Altmetric Badge

Mentioned by

twitter
1 X user
facebook
1 Facebook page

Citations

dimensions_citation
7 Dimensions

Readers on

mendeley
33 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
BaltDC: purification, characterization and infrared spectroscopy of an antiplatelet DC protein isolated from Bothrops alternatus snake venom
Published in
Journal of Venomous Animals and Toxins including Tropical Diseases, July 2017
DOI 10.1186/s40409-017-0126-7
Pubmed ID
Authors

Mariana Santos Matias, Bruna Barbosa de Sousa, Déborah Fernanda da Cunha Pereira, Edigar Henrique Vaz Dias, Carla Cristine Neves Mamede, Mayara Ribeiro de Queiroz, Anielle Christine Almeida Silva, Noelio Oliveira Dantas, Andreimar Martins Soares, Júnia de Oliveira Costa, Fábio de Oliveira

Abstract

Snake venoms are a complex mixture of proteins, organic and inorganic compounds. Some of these proteins, enzymatic or non-enzymatic ones, are able to interact with platelet receptors, causing hemostatic disorders. The possible therapeutic potential of toxins with antiplatelet properties may arouse interest in the pharmacological areas. The present study aimed to purify and characterize an antiplatelet DC protein from Bothrops alternatus snake venom. The protein, called BaltDC (DC protein from B. alternatus snake venom), was purified by a combination of ion-exchange chromatography on DEAE-Sephacel column and gel filtration on Sephadex G-75. The molecular mass was estimated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE). The amino acid sequence of the N-terminal region was carried out by Edman degradation method. Platelet aggregation assays were performed in human platelet-rich plasma (PRP). Infrared (IR) spectroscopy was used in order to elucidate the interactions between BaltDC and platelet membrane. BaltDC ran as a single protein band on SDS-PAGE and showed apparent molecular mass of 32 kDa under reducing or non-reducing conditions. The N-terminal region of the purified protein revealed the amino acid sequence IISPPVCGNELLEVGEECDCGTPENCQNECCDA, which showed identity with other snake venom metalloproteinases (SVMPs). BaltDC was devoid of proteolytic, hemorrhagic, defibrinating or coagulant activities, but it showed a specific inhibitory effect on platelet aggregation induced by ristocetin and epinephrine in PRP. IR analysis spectra strongly suggests that PO3(2-) groups, present in BaltDC, form hydrogen bonds with the PO2(-) groups present in the non-lipid portion of the membrane platelets. BaltDC may be of medical interest since it was able to inhibit platelet aggregation.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 33 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 7 21%
Student > Ph. D. Student 4 12%
Student > Master 3 9%
Researcher 2 6%
Student > Doctoral Student 2 6%
Other 2 6%
Unknown 13 39%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 24%
Biochemistry, Genetics and Molecular Biology 6 18%
Pharmacology, Toxicology and Pharmaceutical Science 2 6%
Veterinary Science and Veterinary Medicine 1 3%
Earth and Planetary Sciences 1 3%
Other 0 0%
Unknown 15 45%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 August 2017.
All research outputs
#19,951,180
of 25,382,440 outputs
Outputs from Journal of Venomous Animals and Toxins including Tropical Diseases
#396
of 539 outputs
Outputs of similar age
#237,699
of 326,762 outputs
Outputs of similar age from Journal of Venomous Animals and Toxins including Tropical Diseases
#9
of 10 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 539 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.6. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 326,762 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one.