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Concentration of Plasmodium falciparum gametocytes in whole blood samples by magnetic cell sorting enhances parasite infection rates in mosquito feeding assays

Overview of attention for article published in Malaria Journal, August 2017
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  • Above-average Attention Score compared to outputs of the same age (52nd percentile)

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Title
Concentration of Plasmodium falciparum gametocytes in whole blood samples by magnetic cell sorting enhances parasite infection rates in mosquito feeding assays
Published in
Malaria Journal, August 2017
DOI 10.1186/s12936-017-1959-9
Pubmed ID
Authors

Isaie J. Reuling, Will J. R. Stone, Marga van de Vegte-Bolmer, Geert-Jan van Gemert, Rianne Siebelink-Stoter, Wouter Graumans, Kjerstin Lanke, Teun Bousema, Robert W. Sauerwein

Abstract

Mosquito-feeding assays are important tools to guide the development and support the evaluation of transmission-blocking interventions. These functional bioassays measure the sporogonic development of gametocytes in blood-fed mosquitoes. Measuring the infectivity of low gametocyte densities has become increasingly important in malaria elimination scenarios. This will pose challenges to the sensitivity and throughput of existing mosquito-feeding assay protocols. Here, different gametocyte concentration methods of blood samples were explored to optimize conditions for detection of positive mosquito infections. Mature gametocytes of Plasmodium falciparum were diluted into whole blood samples of malaria-naïve volunteers. Standard centrifugation, Percoll gradient, magnetic cell sorting (MACS) enrichment were compared using starting blood volumes larger than the control (direct) feed. MACS gametocyte enrichment resulted in the highest infection intensity with statistically significant increases in mean oocyst density in 2 of 3 experiments (p = 0.0003; p ≤ 0.0001; p = 0.2348). The Percoll gradient and standard centrifugation procedures resulted in variable infectivity. A significant increase in the proportion of infected mosquitoes and oocyst density was found when larger volumes of gametocyte-infected blood were used with the MACS procedure. The current study demonstrates that concentration methods of P. falciparum gametocyte-infected whole blood samples can enhance transmission in mosquito-feeding assays. Gametocyte purification by MACS was the most efficient method, allowing the assessment of gametocyte infectivity in low-density gametocyte infections, as can be expected in natural or experimental conditions.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 49 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 12 24%
Student > Master 10 20%
Researcher 10 20%
Other 4 8%
Student > Bachelor 2 4%
Other 4 8%
Unknown 7 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 27%
Biochemistry, Genetics and Molecular Biology 9 18%
Medicine and Dentistry 5 10%
Immunology and Microbiology 4 8%
Engineering 2 4%
Other 6 12%
Unknown 10 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 August 2017.
All research outputs
#12,990,585
of 22,996,001 outputs
Outputs from Malaria Journal
#3,129
of 5,592 outputs
Outputs of similar age
#149,089
of 317,463 outputs
Outputs of similar age from Malaria Journal
#93
of 125 outputs
Altmetric has tracked 22,996,001 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,592 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.8. This one is in the 43rd percentile – i.e., 43% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 317,463 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.
We're also able to compare this research output to 125 others from the same source and published within six weeks on either side of this one. This one is in the 23rd percentile – i.e., 23% of its contemporaries scored the same or lower than it.