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Mapping of leptin and its syntenic genes to chicken chromosome 1p

Overview of attention for article published in BMC Genetics, August 2017
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Title
Mapping of leptin and its syntenic genes to chicken chromosome 1p
Published in
BMC Genetics, August 2017
DOI 10.1186/s12863-017-0543-1
Pubmed ID
Authors

Eyal Seroussi, Frédérique Pitel, Sophie Leroux, Mireille Morisson, Susanne Bornelöv, Shoval Miyara, Sara Yosefi, Larry A. Cogburn, David W. Burt, Leif Anderson, Miriam Friedman-Einat

Abstract

Misidentification of the chicken leptin gene has hampered research of leptin signaling in this species for almost two decades. Recently, the genuine leptin gene with a GC-rich (~70%) repetitive-sequence content was identified in the chicken genome but without indicating its genomic position. This suggests that such GC-rich sequences are difficult to sequence and therefore substantial regions are missing from the current chicken genome assembly. A radiation hybrid panel of chicken-hamster Wg3hCl2 cells was used to map the genome location of the chicken leptin gene. Contrary to our expectations, based on comparative genome mapping and sequence characteristics, the chicken leptin was not located on a microchromosome, which are known to contain GC-rich and repetitive regions, but at the distal tip of the largest chromosome (1p). Following conserved synteny with other vertebrates, we also mapped five additional genes to this genomic region (ARF5, SND1, LRRC4, RBM28, and FLNC), bridging the genomic gap in the current Galgal5 build for this chromosome region. All of the short scaffolds containing these genes were found to consist of GC-rich (54 to 65%) sequences comparing to the average GC-content of 40% on chromosome 1. In this syntenic group, the RNA-binding protein 28 (RBM28) was in closest proximity to leptin. We deduced the full-length of the RBM28 cDNA sequence and profiled its expression patterns detecting a negative correlation (R = - 0.7) between the expression of leptin and of RBM28 across tissues that expressed at least one of the genes above the average level. This observation suggested a local regulatory interaction between these genes. In adipose tissues, we observed a significant increase in RBM28 mRNA expression in breeds with lean phenotypes. Mapping chicken leptin together with a cluster of five syntenic genes provided the final proof for its identification as the true chicken ortholog. The high GC-content observed for the chicken leptin syntenic group suggests that other similar clusters of genes in GC-rich genomic regions are missing from the current genome assembly (Galgal5), which should be resolved in future assemblies of the chicken genome.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 18%
Student > Doctoral Student 3 18%
Student > Master 2 12%
Researcher 2 12%
Student > Bachelor 2 12%
Other 1 6%
Unknown 4 24%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 41%
Biochemistry, Genetics and Molecular Biology 3 18%
Business, Management and Accounting 1 6%
Veterinary Science and Veterinary Medicine 1 6%
Immunology and Microbiology 1 6%
Other 0 0%
Unknown 4 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 November 2017.
All research outputs
#7,942,461
of 13,181,286 outputs
Outputs from BMC Genetics
#407
of 863 outputs
Outputs of similar age
#142,556
of 266,129 outputs
Outputs of similar age from BMC Genetics
#1
of 1 outputs
Altmetric has tracked 13,181,286 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 863 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
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