Title |
Role of transcription regulatory sequence in regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus
|
---|---|
Published in |
Veterinary Research, August 2017
|
DOI | 10.1186/s13567-017-0445-2 |
Pubmed ID | |
Authors |
Chengbao Wang, Han Meng, Yujin Gao, Hui Gao, Kangkang Guo, Fernando Almazan, Isabel Sola, Luis Enjuanes, Yanming Zhang, Levon Abrahamyan |
Abstract |
In order to gain insight into the role of the transcription regulatory sequences (TRSs) in the regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus (PRRSV), the enhanced green fluorescent protein (EGFP) gene, under the control of the different structural gene TRSs, was inserted between the N gene and 3'-UTR of the PRRSV genome and EGFP expression was analyzed for each TRS. TRSs of all the studied structural genes of PRRSV positively modulated EGFP expression at different levels. Among the TRSs analyzed, those of GP2, GP5, M, and N genes highly enhanced EGFP expression without altering replication of PRRSV. These data indicated that structural gene TRSs could be an extremely useful tool for foreign gene expression using PRRSV as a vector. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 18% |
Researcher | 3 | 18% |
Lecturer | 1 | 6% |
Other | 1 | 6% |
Student > Ph. D. Student | 1 | 6% |
Other | 3 | 18% |
Unknown | 5 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Veterinary Science and Veterinary Medicine | 3 | 18% |
Biochemistry, Genetics and Molecular Biology | 3 | 18% |
Agricultural and Biological Sciences | 2 | 12% |
Immunology and Microbiology | 2 | 12% |
Chemistry | 1 | 6% |
Other | 0 | 0% |
Unknown | 6 | 35% |