Title |
Development and antimicrobial susceptibility studies of in vitro monomicrobial and polymicrobial biofilm models with Aspergillus fumigatus and Pseudomonas aeruginosa
|
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Published in |
BMC Microbiology, March 2014
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DOI | 10.1186/1471-2180-14-53 |
Pubmed ID | |
Authors |
Elias K Manavathu, Dora L Vager, Jose A Vazquez |
Abstract |
Mixed microbial infections of the respiratory tracts with P. aeruginosa and A. fumigatus capable of producing biofilms are commonly found in cystic fibrosis patients. The primary objective of this study was to develop an in vitro model for P. aeruginosa and A. fumigatus polymicrobial biofilm to study the efficacy of various antimicrobial drugs alone and in combinations against biofilm-embedded cells. Simultaneous static cocultures of P. aeruginosa and sporelings were used for the development of in vitro P. aeruginosa-A. fumigatus polymicrobial biofilm in SD broth in 24-well cell culture plates at 35°C, and the biofilm formation was monitored microscopically and spectrophotometrically. Using P. aeruginosa-A. fumigatus sporelings cocultures we examined the effects of various antimicrobial drugs alone and in combination against polymicrobial biofilm by CFU and tetrazolium reduction assays. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 1% |
Unknown | 87 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 17 | 19% |
Researcher | 12 | 14% |
Student > Bachelor | 9 | 10% |
Student > Master | 8 | 9% |
Student > Doctoral Student | 7 | 8% |
Other | 16 | 18% |
Unknown | 19 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 27 | 31% |
Immunology and Microbiology | 11 | 13% |
Biochemistry, Genetics and Molecular Biology | 11 | 13% |
Medicine and Dentistry | 5 | 6% |
Engineering | 3 | 3% |
Other | 7 | 8% |
Unknown | 24 | 27% |