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A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454

Overview of attention for article published in Genome Biology, February 2010
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (86th percentile)
  • Above-average Attention Score compared to outputs of the same age and source (62nd percentile)

Mentioned by

patent
38 patents

Citations

dimensions_citation
88 Dimensions

Readers on

mendeley
258 Mendeley
citeulike
9 CiteULike
connotea
3 Connotea
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Title
A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454
Published in
Genome Biology, February 2010
DOI 10.1186/gb-2010-11-2-r15
Pubmed ID
Authors

Niall J Lennon, Robert E Lintner, Scott Anderson, Pablo Alvarez, Andrew Barry, William Brockman, Riza Daza, Rachel L Erlich, Georgia Giannoukos, Lisa Green, Andrew Hollinger, Cindi A Hoover, David B Jaffe, Frank Juhn, Danielle McCarthy, Danielle Perrin, Karen Ponchner, Taryn L Powers, Kamran Rizzolo, Dana Robbins, Elizabeth Ryan, Carsten Russ, Todd Sparrow, John Stalker, Scott Steelman, Michael Weiand, Andrew Zimmer, Matthew R Henn, Chad Nusbaum, Robert Nicol

Abstract

We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 258 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 19 7%
Brazil 4 2%
Switzerland 2 <1%
Australia 2 <1%
Germany 2 <1%
Spain 2 <1%
United Kingdom 1 <1%
Canada 1 <1%
Belgium 1 <1%
Other 5 2%
Unknown 219 85%

Demographic breakdown

Readers by professional status Count As %
Researcher 100 39%
Student > Ph. D. Student 55 21%
Professor 19 7%
Other 17 7%
Professor > Associate Professor 16 6%
Other 33 13%
Unknown 18 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 175 68%
Biochemistry, Genetics and Molecular Biology 29 11%
Medicine and Dentistry 6 2%
Environmental Science 5 2%
Computer Science 4 2%
Other 14 5%
Unknown 25 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 April 2024.
All research outputs
#3,798,287
of 25,373,627 outputs
Outputs from Genome Biology
#2,558
of 4,467 outputs
Outputs of similar age
#19,281
of 172,987 outputs
Outputs of similar age from Genome Biology
#9
of 24 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,467 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one is in the 42nd percentile – i.e., 42% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 172,987 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 24 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.