Title |
Superior triacylglycerol (TAG) accumulation in starchless mutants of Scenedesmus obliquus: (I) mutant generation and characterization
|
---|---|
Published in |
Biotechnology for Biofuels and Bioproducts, May 2014
|
DOI | 10.1186/1754-6834-7-69 |
Pubmed ID | |
Authors |
Lenny de Jaeger, Ruben EM Verbeek, René B Draaisma, Dirk E Martens, Jan Springer, Gerrit Eggink, René H Wijffels |
Abstract |
Microalgae are a promising platform for producing neutral lipids, to be used in the application for biofuels or commodities in the feed and food industry. A very promising candidate is the oleaginous green microalga Scenedesmus obliquus, because it accumulates up to 45% w/w triacylglycerol (TAG) under nitrogen starvation. Under these conditions, starch is accumulated as well. Starch can amount up to 38% w/w under nitrogen starvation, which is a substantial part of the total carbon captured. When aiming for optimized TAG production, blocking the formation of starch could potentially increase carbon allocation towards TAG. In an attempt to increase TAG content, productivity and yield, starchless mutants of this high potential strain were generated using UV mutagenesis. Previous studies in Chlamydomonas reinhardtii have shown that blocking the starch synthesis yields higher TAG contents, although these TAG contents do not surpass those of oleaginous microalgae yet. So far no starchless mutants in oleaginous green microalgae have been isolated that result in higher TAG productivities. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 33% |
United States | 1 | 33% |
Netherlands | 1 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Netherlands | 1 | <1% |
Italy | 1 | <1% |
Unknown | 194 | 99% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 33 | 17% |
Student > Master | 28 | 14% |
Student > Bachelor | 28 | 14% |
Researcher | 21 | 11% |
Student > Doctoral Student | 15 | 8% |
Other | 29 | 15% |
Unknown | 42 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 50 | 26% |
Biochemistry, Genetics and Molecular Biology | 37 | 19% |
Chemical Engineering | 12 | 6% |
Engineering | 12 | 6% |
Environmental Science | 7 | 4% |
Other | 22 | 11% |
Unknown | 56 | 29% |