Title |
An efficient method for stable protein targeting in grasses (Poaceae): a case study in Puccinellia tenuiflora
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Published in |
BMC Biotechnology, June 2014
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DOI | 10.1186/1472-6750-14-52 |
Pubmed ID | |
Authors |
Yuanyuan Bu, Mengqing Zhao, Bo Sun, Xinxin Zhang, Tetsuo Takano, Shenkui Liu |
Abstract |
An efficient transformation method is lacking for most non-model plant species to test gene function. Therefore, subcellular localization of proteins of interest from non-model plants is mainly carried out through transient transformation in homologous cells or in heterologous cells from model species such as Arabidopsis. Although analysis of expression patterns in model organisms like yeast and Arabidopsis can provide important clues about protein localization, these heterologous systems may not always faithfully reflect the native subcellular distribution in other species. On the other hand, transient expression in protoplasts from species of interest has limited ability for detailed sub-cellular localization analysis (e.g., those involving subcellular fractionation or sectioning and immunodetection), as it results in heterogeneous populations comprised of both transformed and untransformed cells. |
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France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Unknown | 10 | 100% |
Demographic breakdown
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Student > Bachelor | 2 | 20% |
Researcher | 2 | 20% |
Other | 1 | 10% |
Professor | 1 | 10% |
Student > Doctoral Student | 1 | 10% |
Other | 2 | 20% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 5 | 50% |
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Social Sciences | 1 | 10% |
Unknown | 2 | 20% |