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The effect of storage at ambient temperature on the feline fecal microbiota

Overview of attention for article published in BMC Veterinary Research, August 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • High Attention Score compared to outputs of the same age and source (94th percentile)

Mentioned by

blogs
1 blog
twitter
13 tweeters
facebook
2 Facebook pages

Citations

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16 Dimensions

Readers on

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44 Mendeley
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Title
The effect of storage at ambient temperature on the feline fecal microbiota
Published in
BMC Veterinary Research, August 2017
DOI 10.1186/s12917-017-1188-z
Pubmed ID
Authors

Moran Tal, Adronie Verbrugghe, Diego E. Gomez, Charlotte Chau, J. Scott Weese

Abstract

Feline fecal microbiota analyses can potentially be impacted by a variety of factors such as sample preparation, sequencing method and bioinformatics analyses. Another potential influence is changes in the microbiota from storage of samples prior to processing. This study examined the effect of ambient temperature exposure on the feline fecal microbiota composition. Fecal samples were collected from 12 healthy cats, within 15 min after defecation. Samples were aliquoted and the first aliquot was frozen at -80 °C within 1 hour of defecation. Remaining aliquots were maintained at ambient temperature (20 to 23 °C) and frozen at -80 °C at 6, 12, 24, 36, 48, 72 and 96 h after collection. DNA was extracted from all aliquots, and polymerase chain reaction (PCR). The PCR products were sequenced with next-generation sequencing (Illumina MiSeq). No significant differences were observed in alpha and beta biodiversity indexes, as well as relative abundance of different taxa over time (P > 0.05 for all tests between time points). Principal coordinate analyses demonstrated that samples cluster mainly by cat, with no significant differences between time points (AMOVA, P > 0.05; HOMOVA, P > 0.05). Linear discriminant analysis effect size method was performed and failed to detect any enriched taxa, between time points. Random forest algorithm analysis indicated homogeneity across time points. Although existing evidence from human fecal storage studies is contradictory, a recent study in companion animals agreed with the current study, demonstrating that maintenance of feline fecal samples at ambient temperature for up to 4 days has no effect on the bacterial membership and structure.

Twitter Demographics

The data shown below were collected from the profiles of 13 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 44 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 11 25%
Other 6 14%
Student > Master 5 11%
Student > Ph. D. Student 4 9%
Student > Bachelor 1 2%
Other 5 11%
Unknown 12 27%
Readers by discipline Count As %
Medicine and Dentistry 10 23%
Veterinary Science and Veterinary Medicine 8 18%
Biochemistry, Genetics and Molecular Biology 5 11%
Agricultural and Biological Sciences 3 7%
Immunology and Microbiology 3 7%
Other 2 5%
Unknown 13 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 September 2018.
All research outputs
#2,251,812
of 22,999,744 outputs
Outputs from BMC Veterinary Research
#138
of 3,064 outputs
Outputs of similar age
#45,450
of 318,830 outputs
Outputs of similar age from BMC Veterinary Research
#4
of 77 outputs
Altmetric has tracked 22,999,744 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,064 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 318,830 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 77 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 94% of its contemporaries.