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Schistosoma japonicum: The design and experimental evaluation of a multivalent DNA vaccine

Overview of attention for article published in Cellular & Molecular Biology Letters, January 2006
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Title
Schistosoma japonicum: The design and experimental evaluation of a multivalent DNA vaccine
Published in
Cellular & Molecular Biology Letters, January 2006
DOI 10.2478/s11658-006-0036-0
Pubmed ID
Authors

Chunyan Li, Longjiang Yu, Zhi Liu, Lu Zhu, Yuan Hu, Min Zhu, Xiaohua Zhu, Youen Shi, Sijin Meng

Abstract

The aim of this study was to construct and evaluate the immunity efficacy of the DNA multivalent vaccine pVIVO(2)SjFABP-23. The vaccine was constructed and produced as follows. Forty BALB/c mice were divided into four groups designated pVIVO(2), pVIVO(2)Sj23, pVIVO(2)SjFABP and pVIVO(2)SjFABP-23. Each mouse was immunized with 100 mug of the corresponding plasmid DNA by intramuscular injection. 28 days post-vaccination, the mice were challenged with S. japonicum cercariae, and the worm and egg burdens were determined 42 days post-challenge. Serum samples were collected from all the mice before and after vaccination and at the end of the experiment, and used for antibody detection. The IFN-gamma and IL-4 levels were quantified in the supernatants of specifically stimulated spleen cells. The number of worms was reduced by 52%, 40% and 42% in mice respectively immunized with pVIVO(2)SjFABP-23, pVIVO(2)Sj23 or pVIVO(2)SjFABP. A respective 61%, 38% and 39% egg reduction was determined relative to those mice that only received the empty pVIVO2 plasmid. pVIVO(2)SjFABP-23 immunization increased IgG levels against SWAP and SEA. Increased IFN-gamma levels were detected in the supernatant of specific stimulated spleen cells from mice immunized with the 3 different constructs. The multivalent DNA vaccine developed induced higher levels of protection than the two monovalent tested vaccines.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 15%
Student > Ph. D. Student 2 15%
Student > Doctoral Student 1 8%
Student > Bachelor 1 8%
Student > Master 1 8%
Other 1 8%
Unknown 5 38%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 23%
Biochemistry, Genetics and Molecular Biology 2 15%
Mathematics 1 8%
Nursing and Health Professions 1 8%
Immunology and Microbiology 1 8%
Other 0 0%
Unknown 5 38%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 June 2014.
All research outputs
#11,149,838
of 12,533,815 outputs
Outputs from Cellular & Molecular Biology Letters
#136
of 174 outputs
Outputs of similar age
#157,193
of 189,955 outputs
Outputs of similar age from Cellular & Molecular Biology Letters
#1
of 3 outputs
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