Title |
Mapping-by-sequencing accelerates forward genetics in barley
|
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Published in |
Genome Biology, June 2014
|
DOI | 10.1186/gb-2014-15-6-r78 |
Pubmed ID | |
Authors |
Martin Mascher, Matthias Jost, Joel-Elias Kuon, Axel Himmelbach, Axel Aßfalg, Sebastian Beier, Uwe Scholz, Andreas Graner, Nils Stein |
Abstract |
Mapping-by-sequencing has emerged as a powerful technique for genetic mapping in several plant and animal species. As this resequencing-based method requires a reference genome, its application to complex plant genomes with incomplete and fragmented sequence resources remains challenging. We perform exome sequencing of phenotypic bulks of a mapping population of barley segregating for a mutant phenotype that increases the rate of leaf initiation. Read depth analysis identifies a candidate gene, which is confirmed by the analysis of independent mutant alleles. Our method illustrates how the genomic resources of barley together with exome resequencing can underpin mapping-by-sequencing. |
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Geographical breakdown
Country | Count | As % |
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Netherlands | 1 | 17% |
India | 1 | 17% |
Switzerland | 1 | 17% |
United Kingdom | 1 | 17% |
Unknown | 2 | 33% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 5 | 83% |
Science communicators (journalists, bloggers, editors) | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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India | 2 | <1% |
Netherlands | 1 | <1% |
France | 1 | <1% |
Germany | 1 | <1% |
Brazil | 1 | <1% |
Sweden | 1 | <1% |
United Kingdom | 1 | <1% |
Argentina | 1 | <1% |
China | 1 | <1% |
Other | 3 | 1% |
Unknown | 222 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 67 | 29% |
Student > Ph. D. Student | 56 | 24% |
Student > Master | 28 | 12% |
Student > Doctoral Student | 14 | 6% |
Student > Bachelor | 11 | 5% |
Other | 28 | 12% |
Unknown | 31 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 149 | 63% |
Biochemistry, Genetics and Molecular Biology | 34 | 14% |
Computer Science | 6 | 3% |
Engineering | 3 | 1% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | <1% |
Other | 5 | 2% |
Unknown | 37 | 16% |