Title |
Protocol: a beginner’s guide to the analysis of RNA-directed DNA methylation in plants
|
---|---|
Published in |
Plant Methods, June 2014
|
DOI | 10.1186/1746-4811-10-18 |
Pubmed ID | |
Authors |
Huiming Zhang, Kai Tang, Bangshing Wang, Cheng-Guo Duan, Zhaobo Lang, Jian-Kang Zhu |
Abstract |
DNA methylation is a conserved epigenetic mark that controls genome stability, development and environmental responses in many eukaryotes. DNA methylation can be guided by non-coding RNAs that include small interfering RNAs and scaffold RNAs. Although measurement of DNA methylation and regulatory non-coding RNAs is desirable for many biologists who are interested in exploring epigenetic regulation in their areas, conventional methods have limitations and are technically challenging. For instance, traditional siRNA detection through RNA hybridization requires relatively large amount of small RNAs and involves radioactive isotopes. An alternative approach is RT-qPCR that employs stem loop primers during reverse transcription; however, it requires a prerequisite that the exact sequences of siRNAs should be known. |
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United Kingdom | 3 | 75% |
France | 1 | 25% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 25% |
Scientists | 1 | 25% |
Mendeley readers
Geographical breakdown
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Norway | 1 | <1% |
Sweden | 1 | <1% |
Czechia | 1 | <1% |
United Kingdom | 1 | <1% |
United States | 1 | <1% |
Philippines | 1 | <1% |
Unknown | 131 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 35 | 26% |
Student > Ph. D. Student | 29 | 21% |
Student > Master | 17 | 12% |
Student > Bachelor | 14 | 10% |
Student > Doctoral Student | 8 | 6% |
Other | 14 | 10% |
Unknown | 20 | 15% |
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Environmental Science | 1 | <1% |
Other | 2 | 1% |
Unknown | 21 | 15% |