Title |
Directed evolution of a filamentous fungus for thermotolerance
|
---|---|
Published in |
BMC Biotechnology, August 2009
|
DOI | 10.1186/1472-6750-9-74 |
Pubmed ID | |
Authors |
Eudes de Crecy, Stefan Jaronski, Benjamin Lyons, Thomas J Lyons, Nemat O Keyhani |
Abstract |
Filamentous fungi are the most widely used eukaryotic biocatalysts in industrial and chemical applications. Consequently, there is tremendous interest in methodology that can use the power of genetics to develop strains with improved performance. For example, Metarhizium anisopliae is a broad host range entomopathogenic fungus currently under intensive investigation as a biologically based alternative to chemical pesticides. However, it use is limited by the relatively low tolerance of this species to abiotic stresses such as heat, with most strains displaying little to no growth between 35-37 degrees C. In this study, we used a newly developed automated continuous culture method called the Evolugator, which takes advantage of a natural selection-adaptation strategy, to select for thermotolerant variants of M. anisopliae strain 2575 displaying robust growth at 37 degrees C. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 10% |
Sierra Leone | 1 | 10% |
Bosnia and Herzegovina | 1 | 10% |
Unknown | 7 | 70% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 8 | 80% |
Scientists | 2 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 1% |
France | 2 | <1% |
Colombia | 1 | <1% |
Germany | 1 | <1% |
Brazil | 1 | <1% |
India | 1 | <1% |
Netherlands | 1 | <1% |
Belgium | 1 | <1% |
Slovakia | 1 | <1% |
Other | 2 | <1% |
Unknown | 187 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 58 | 29% |
Researcher | 28 | 14% |
Student > Master | 23 | 11% |
Student > Bachelor | 18 | 9% |
Student > Doctoral Student | 8 | 4% |
Other | 29 | 14% |
Unknown | 37 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 91 | 45% |
Biochemistry, Genetics and Molecular Biology | 21 | 10% |
Immunology and Microbiology | 9 | 4% |
Environmental Science | 7 | 3% |
Chemistry | 5 | 2% |
Other | 24 | 12% |
Unknown | 44 | 22% |