Title |
Distinct HIV-1 entry phenotypes are associated with transmission, subtype specificity, and resistance to broadly neutralizing antibodies
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Published in |
Retrovirology, June 2014
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DOI | 10.1186/1742-4690-11-48 |
Pubmed ID | |
Authors |
Kelechi Chikere, Nicholas E Webb, Tom Chou, Katharina Borm, Jasminka Sterjovski, Paul R Gorry, Benhur Lee |
Abstract |
The efficiency of CD4/CCR5 mediated HIV-1 entry has important implications for pathogenesis and transmission. The HIV-1 receptor affinity profiling (Affinofile) system analyzes and quantifies the infectivity of HIV-1 envelopes (Envs) across a spectrum of CD4/CCR5 expression levels and distills these data into a set of Affinofile metrics. The Affinofile system has shed light on how differential CD4/CCR5 usage efficiencies contributes to an array of Env phenotypes associated with cellular tropism, viral pathogenesis, and CCR5 inhibitor resistance. To facilitate more rapid, convenient, and robust analysis of HIV-1 entry phenotypes, we engineered a reporter Affinofile system containing a Tat- and Rev-dependent Gaussia luciferase-eGFP-Reporter (GGR) that is compatible with the use of pseudotyped or replication competent viruses with or without a virally encoded reporter gene. This GGR Affinofile system enabled a higher throughput characterization of CD4/CCR5 usage efficiencies associated with differential Env phenotypes. |
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Geographical breakdown
Country | Count | As % |
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Venezuela, Bolivarian Republic of | 1 | 33% |
United Kingdom | 1 | 33% |
Unknown | 1 | 33% |
Demographic breakdown
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
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South Africa | 1 | 2% |
Unknown | 40 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 12 | 29% |
Student > Master | 7 | 17% |
Student > Bachelor | 4 | 10% |
Student > Ph. D. Student | 3 | 7% |
Professor | 2 | 5% |
Other | 4 | 10% |
Unknown | 9 | 22% |
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Immunology and Microbiology | 9 | 22% |
Biochemistry, Genetics and Molecular Biology | 5 | 12% |
Medicine and Dentistry | 3 | 7% |
Social Sciences | 1 | 2% |
Other | 3 | 7% |
Unknown | 8 | 20% |